Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1060722
View all NPRB/NPR2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human NPRB/NPR2
Arg23-Ile458
Accession # P20594

Specificity

Detects human NPRB/NPR2 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NPRB/NPR2 Antibody

Detection of NPRB/NPR2 in HEK293 cells transfected with Human NPR2 and eGFP by Flow Cytometry.

HEK293 cells transfected with Human NPR2 and eGFP were stained with either (A) Mouse Anti-Human NPRB/NPR2 Monoclonal Antibody (Catalog # MAB9725) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NPRB/NPR2 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 cells transfected with Human NPR2 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NPRB/NPR2

Human Natriuretic Peptide Receptor-2 (NPR2), also known as NPRB, ANP-RB or guanylyl Cyclase-B, is a member of the guanylyl cyclase family of proteins. NPR2 is a type I transmembrane glycoprotein that contains a 436 amino acid extracellular domain (ECD) (aa 23‑458) for ligand binding, and a 569 amino acid cytoplasmic domain that contains both a protein kinase domain and a carboxyl-terminal guanylate cyclase domain. NPR2 is expressed most highly in in bone, brain, fibroblasts, heart, kidney, liver, lung, uterine, and vascular smooth muscle tissue (1). NPR2 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B type natriuretic peptide), and NPR2 is the principal receptor of CNP (C type natriuretic peptide) (1, 2). Ligand binding to the extracellular ligand binding domain, plus ATP to the intracellular kinase domain activates a cytoplasmic guanylate cyclase (2). NPR2 pathway play a critical role in regulation of skeletal growth (3), and patients with single defect NPR2 alleles are statistically shorter than the average population (4). Over the extracellular domain, human NPR2 is 97% and 96% identical to mouse and rat NPR2, respectively.

References

  1. Potter, L.R. et al. (2009) Handb Exp Pharmacol 191:341.
  2. Chang, M.S. et al. (1989) Nature 341:68.
  3. Tsuji, T. and Kunieda T. (2005) J Biol Chem 280:14288.
  4. Olney, R.C. et al. (2006) J Clin Endocrinol Metab 91:1229.

Long Name

Natriuretic Peptide Receptor B

Alternate Names

AMDM, ANPb, ANPRB, ECDM, GC-B, GUC2B, NPR2, NPRBi, SNSK

Entrez Gene IDs

4882 (Human); 230103 (Mouse); 116564 (Rat)

Gene Symbol

NPR2

UniProt

P20594

Additional NPRB/NPR2 Products

Product Documents for Human NPRB/NPR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human NPRB/NPR2 Antibody

For research use only

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