|Cell Proliferation Induced by NRG1 Isoform SMDF and Neutralization by Human NRG1 Isoform SMDF Antibody. Recombinant Human NRG1 Isoform SMDF (Catalog # 378‑SM) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1 Isoform SMDF (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1 Isoform SMDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF378). The ND50 is typically 2‑8 µg/mL.|
The heregulin (also known as neuregulin) family of cytokines is comprised of multiple secreted or membrane-bound isoforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. All heregulin family members share an epidermal growth factor (EGF)-like domain ( alpha - or beta ‑variant) that interacts with the erbB family of tyrosine kinase receptors. NRG1 Isoform SMDF is a heregulin isoform containing a C-terminal EGF-like domain ( beta ‑variant) and a unique N-terminal sequence that lacks an Ig-like domain which is present in all other known heregulins. NRG1 Isoform SMDF also lacks a transmembrane domain and the cytoplasmic tail. NRG1 Isoform SMDF expression has been found to be restricted to the nervous system. It is likely that NRG1 Isoform SMDF may play an important role in neural-specific functions.
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