Detects human NRG1 Isoform SMDF in direct ELISAs and Western blots. Neutralizes 60‑80% of the biological activity of human NRG1 Isoform SMDF.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NRG1 Isoform SMDF Met1-Glu296 Accession # NP_039253
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human NRG1 Isoform SMDF (Catalog # 378-SM)
Measured by its ability to neutralize NRG1 Isoform SMDF-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 2‑8 µg/mL in the presence of 12 ng/mL Recombinant Human NRG1 Isoform SMDF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by NRG1 Isoform SMDF and Neutralization by Human NRG1 Isoform SMDF Antibody. Recombinant Human NRG1 Isoform SMDF (Catalog # 378‑SM) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1 Isoform SMDF (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1 Isoform SMDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF378). The ND50 is typically 2‑8 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-1 Isoform SMDF
The heregulin (also known as neuregulin) family of cytokines is comprised of multiple secreted or membrane-bound isoforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. All heregulin family members share an epidermal growth factor (EGF)-like domain ( alpha - or beta ‑variant) that interacts with the erbB family of tyrosine kinase receptors. NRG1 Isoform SMDF is a heregulin isoform containing a C-terminal EGF-like domain ( beta ‑variant) and a unique N-terminal sequence that lacks an Ig-like domain which is present in all other known heregulins. NRG1 Isoform SMDF also lacks a transmembrane domain and the cytoplasmic tail. NRG1 Isoform SMDF expression has been found to be restricted to the nervous system. It is likely that NRG1 Isoform SMDF may play an important role in neural-specific functions.
Yarden, Y. and D. Wen (1994) in Guidebook to Cytokines and Their Receptors,
N.A. Nicola, Ed., Oxford University Press, p. 146.
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