Human NRL Antibody

Detection of NRL by Western Blot

Neural retina leucine–RNA-binding protein (NRL–RBP) interactors are enriched in R-loop proteins.(A) Western blot of GST-NRL affinity-purified RBPs from bovine retina. Benzonase was added to retinal lysates during GST-NRL incubations. (B) Protein–protein interaction (PPI) network showing RBP experimental interactions from String. The edge thickness represents the confidence score with a cutoff of 0.4. Name of proteins identified in 2/4 assays are shown. Proteins identified in three out of four assays are highlighted in red. (C) Venn diagram showing intersection of R-loop proteomes common to four studies (Li et al., 2020; Mosler et al., 2021; Wang et al., 2018; Padmanabhan et al., 2012) and NRL–RBP candidate interactors identified in 2/4 assays.Figure 1—figure supplement 1—source data 1.PDF file containing original western blots for Figure 1B, C, Figure 1—figure supplement 1A indicating the relevant bands. Figure 1—figure supplement 1—source data 2.Original files for western blots displayed in Figure 1B, C, Figure 1—figure supplement 1A.PDF file containing original western blots for Figure 1B, C, Figure 1—figure supplement 1A indicating the relevant bands. Original files for western blots displayed in Figure 1B, C, Figure 1—figure supplement 1A. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40047526), licensed under a CC-BY license. Not internally tested by R&D Systems.