Human NT-3 Propeptide Antibody

Catalog # Availability Size / Price Qty
AF3056
AF3056-SP
Product Details
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Reviews (1)

Human NT-3 Propeptide Antibody Summary

Species Reactivity
Human
Specificity
Detects human NT-3 Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse NT-3 Propeptide is observed, 2% cross-reactivity with recombinant human NT-4 propeptide is observed, and less than 1% cross-reactivity with recombinant human NT-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NT-3
Asn19-Arg138
Accession # P20783
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NT‑3 (Catalog # 267-N3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NT-3

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

References
  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Neurotrophin 3
Entrez Gene IDs
4908 (Human)
Alternate Names
HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human NT-3 Propeptide Antibody
By Anonymous on 05/18/2016
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human

Over expression of proNT3 in 293 HEK cells.