Human NT-4 Propeptide Antibody

R&D Systems | Catalog # AF3450

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NT-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NT‑4 Propeptide
Gln25-Arg80
Accession # P34130

Specificity

Detects human NT-4 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human NT-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NT-4 Propeptide Antibody

Detection of Human NT‑4 Propeptide antibody by Western Blot.

Detection of Human NT‑4 Propeptide by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line, LNCaP human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NT-4 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3450) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NT-4 Propeptide at approximately 36 kDa (corresponent to proform). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human NT-4 Propeptide Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: PC‑3 human prostate cancer cell line, LNCaP human prostate cancer cell line, and DU145 human prostate carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NT-4

NT-4 Propeptide (neurotrophin-4/5) is a 27 kDa, secreted, glycosylated neuronal polypeptide that belongs to the NGF-beta family of neurotrophic factors. It is synthesized as a proform that contains a 9 kDa, 56 amino acid (aa) proregion (aa 25-80) and a 130 aa mature segment. The mature molecule forms both nondisulfide linked heterodimers (with NGF) and homodimers. It is unclear if 186 aa NT-4 Propeptide dimerizes, but this might be anticipated. Proneurotrophins are believed to activate NGFR and sortilin complexes and neurotrophin proregions regulate neurotrophin maturation. Over aa 25-80, human NT-4 Propeptide is 89% and 91% aa identical to mouse and canine NT-4 Propeptide, respectively.

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Entrez Gene IDs

4909 (Human)

Gene Symbol

NTF4

UniProt

P34130

Additional NT-4 Products

Product Documents for Human NT-4 Propeptide Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NT-4 Propeptide Antibody

For research use only

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