Human NT-4 Propeptide Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human NT-4 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human NT-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human NT‑4 Propeptide
    Gln25-Arg80
    Accession # P34130
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human NT‑4 Propeptide by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line, LNCaP human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NT‑4 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3450) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NT‑4 Propeptide at approximately 36 kDa (corresponent to proform). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NT-4

NT-4 Propeptide (neurotrophin-4/5) is a 27 kDa, secreted, glycosylated neuronal polypeptide that belongs to the NGF-beta family of neurotrophic factors. It is synthesized as a proform that contains a 9 kDa, 56 amino acid (aa) proregion (aa 25-80) and a 130 aa mature segment. The mature molecule forms both nondisulfide linked heterodimers (with NGF) and homodimers. It is unclear if 186 aa NT-4 Propeptide dimerizes, but this might be anticipated. Proneurotrophins are believed to activate NGFR and sortilin complexes and neurotrophin proregions regulate neurotrophin maturation. Over aa 25-80, human NT-4 Propeptide is 89% and 91% aa identical to mouse and canine NT-4 Propeptide, respectively.

  • Long Name:
    Neurotrophin 4
  • Entrez Gene IDs:
    4909 (Human)
  • Alternate Names:
    GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 4; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; neurotrophin-5; Neutrophic factor 4; NT4; NT-4; NT-4/5; NT-5; NTF4; NTF5; NTF5NT5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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