Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NT-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NT‑4 Propeptide
Gln25-Arg80
Accession # P34130

Specificity

Detects human NT-4 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human NT-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NT‑4 Propeptide Antibody

Detection of Human NT‑4 Propeptide antibody by Western Blot.

Detection of Human NT‑4 Propeptide by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line, LNCaP human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NT-4 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3450) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NT-4 Propeptide at approximately 36 kDa (corresponent to proform). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human NT‑4 Propeptide Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: PC‑3 human prostate cancer cell line, LNCaP human prostate cancer cell line, and DU145 human prostate carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NT-4

NT-4 Propeptide (neurotrophin-4/5) is a 27 kDa, secreted, glycosylated neuronal polypeptide that belongs to the NGF-beta family of neurotrophic factors. It is synthesized as a proform that contains a 9 kDa, 56 amino acid (aa) proregion (aa 25-80) and a 130 aa mature segment. The mature molecule forms both nondisulfide linked heterodimers (with NGF) and homodimers. It is unclear if 186 aa NT-4 Propeptide dimerizes, but this might be anticipated. Proneurotrophins are believed to activate NGFR and sortilin complexes and neurotrophin proregions regulate neurotrophin maturation. Over aa 25-80, human NT-4 Propeptide is 89% and 91% aa identical to mouse and canine NT-4 Propeptide, respectively.

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Entrez Gene IDs

4909 (Human)

Gene Symbol

NTF4

UniProt

P34130

Additional NT-4 Products

Product Documents for Human NT‑4 Propeptide Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NT‑4 Propeptide Antibody

For research use only

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