Human Occludin Antibody (690213)

R&D Systems | Catalog # MAB7074

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 690213
View all Occludin Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Occludin
Accession # Q16625

Specificity

Detects human Occludin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Occludin Antibody (690213)

Occludin antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

Occludin in HUVEC Human Cells.

Occludin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Occludin Monoclonal Antibody (Catalog # MAB7074) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Occludin by Western Blot

Detection of Human Occludin by Western Blot

Analysis of barrier formation in endothelial monolayer.(A) Expression of endothelial junctional molecules CD31, CD144 and ZO-1 (from left to right) and actin (far right) in hCMVEC monolayer 48 h after seeding. (B) The permeability of hCMVEC monolayer to FITC-dextran 48 h after seeding (mean ± SEM, n = 4). (C) Time course analysis of endothelial tight junction molecule occludin expression at 24, 48 and 72 h post seeding of hCMVECs using Western blot using a mouse monoclonal anti-occludin (left) and rabbit polyclonal anti-occludin (right). (D) Real time measurement of electrical resistance across the endothelial monolayer as an indicator of endothelial barrier integrity. The barrier resistance (Rb) and the basolateral adhesion (Alpha) due to focal adhesions can be modelled using the ECIS Z theta software when the experiment is run using multi-frequency mode (250 to 64000 Hz). The modelling reveals that the basolateral adhesion occurs very fast and junction formation begins around 10 hours after seeding. Overall barrier resistance is the summation of both Rb and alpha, but this software function can determine whether barrier changes are predominantly due to the Rb. Data are mean ± SEM (n = 4). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0180267), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Occludin Antibody (690213)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 2 reviews rated 5 using MAB7074 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Occludin

Occludin is an integral membrane protein with an apparent molecular mass of approximately 65 kDa.  It is localized exclusively at tight junctions (TJ) of select epithelial and endothelial cells.  The protein is 522 amino acids (aa) in length and contains a cytoplasmic N-terminus, four transmembrane domains, and a long COOH‑terminal cytoplasmic domain (domain E) that contains 255 aa.  Residues 1-66 make up the first cytoplasmic domain, and that is included in the MARVEL region consisting of aa 57-210.  The MARVEL region is a membrane-associating domain that spans aa 67-243.  Of note, residues 92-131 are glycine and tyrosine rich.  Residues 244-265 constitute the last transmembrane region, with aa 266-522 representing the long cytoplasmic domain termed domain E.  At the TJ, Occludin associates with membrane peripheral protein ZO-1 (220 kDa).  Human Occludin shares 90% aa sequence identity with mouse Occludin.

Alternate Names

BLCPMG, OCLN

Entrez Gene IDs

100506658 (Human); 18260 (Mouse); 83497 (Rat)

Gene Symbol

OCLN

UniProt

Q16625

Additional Occludin Products

Product Documents for Human Occludin Antibody (690213)

Certificate of Analysis

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Product Specific Notices for Human Occludin Antibody (690213)

For research use only

Citations for Human Occludin Antibody (690213)

Customer Reviews for Human Occludin Antibody (690213) (2)

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  • Human Occludin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig SC cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    MAB7074 Occludin antibody label pig SC cells with alexa488
    Human Occludin Antibody (690213) MAB7074
  • Human Occludin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Hep2G cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    MAB7074 Occludin antibody labeling Hep2G cells with Alexa 488
    Human Occludin Antibody (690213) MAB7074

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