Occludin in HUVEC Human Cells.
Occludin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Occludin Monoclonal Antibody (Catalog # MAB7074) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Occludin is an integral membrane protein with an apparent molecular mass of approximately 65 kDa. It is localized exclusively at tight junctions (TJ) of select epithelial and endothelial cells. The protein is 522 amino acids (aa) in length and contains a cytoplasmic N-terminus, four transmembrane domains, and a long COOH‑terminal cytoplasmic domain (domain E) that contains 255 aa. Residues 1-66 make up the first cytoplasmic domain, and that is included in the MARVEL region consisting of aa 57-210. The MARVEL region is a membrane-associating domain that spans aa 67-243. Of note, residues 92-131 are glycine and tyrosine rich. Residues 244-265 constitute the last transmembrane region, with aa 266-522 representing the long cytoplasmic domain termed domain E. At the TJ, Occludin associates with membrane peripheral protein ZO-1 (220 kDa). Human Occludin shares 90% aa sequence identity with mouse Occludin.
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