Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 893753
View all OCTN2/SLC22A5 Primary Antibodies »

Product Specifications

Immunogen

NS0 transfected with human SLC22A5
Accession # O76082

Specificity

Detects human OCTN2/SLC22A5 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human OCTN2/SLC22A5 Antibody

Detection of OCTN2/SLC22A5 in HEK293 Human Cell Line Transfected with Human OCTN2/SLC22A5 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with (A) human OCTN2/SLC22A5 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human OCTN2/SLC22A5 Monoclonal Antibody (Catalog # MAB11004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human OCTN2/SLC22A5 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human OCTN2/SLC22A5 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OCTN2/SLC22A5

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for the elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A5 is an integral plasma membrane protein which functions as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. Mutations in SLC22A5 are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy.

Long Name

Solute Carrier Family 22 (Organic Cation/Carnitin Transporter) Member 5

Alternate Names

CDSP, OCTN2VT, SCD, SLC22A5

Entrez Gene IDs

6584 (Human); 20520 (Mouse); 29726 (Rat)

Gene Symbol

SLC22A5

UniProt

O76082 (Human)

Additional OCTN2/SLC22A5 Products

Product Documents for Human OCTN2/SLC22A5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human OCTN2/SLC22A5 Antibody

For research use only

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