Detection of Human Olfactomedin-4/OLFM4 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Olfactomedin-4/OLFM4 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(OLFM4), previously called GC-1 (G-CSF-stimulated clone-1), GW112 or hOlfD, is
a 64 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin
family of proteins. Mature Olfactomedin-4 is a 490 amino acid (aa) protein that
is secreted mainly as multimers. Human Olfactomedin-4 is mainly expressed by
gastrointestinal stem cells, developing granulocytes, type M4 acute myelogenous
leukemias, and intestinal and prostate cancer cells and is upregulated by NF-kB signaling. It is
reported to facilitate cell-cell adhesion by binding to lectins and cadherins,
and to be anti-apoptotic. Mature human Olfactomedin-4 shares 71% aa sequence
identity with mouse and rat Olfactomedin-4.
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