Human Olfactomedin-4/OLFM4 Antibody

Catalog # Availability Size / Price Qty
MAB9218-100
MAB9218-SP
Detection  of HumanOlfactomedin-4/OLFM4 by Western Blot.
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Human Olfactomedin-4/OLFM4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Olfactomedin-4/OLFM4 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 806305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Olfactomedin-4/OLFM4
Asp21-Gln510
Accession # Q6UX06
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of HumanOlfactomedin-4/OLFM4 by Western Blot. View Larger

Detection of Human
Olfactomedin-4/OLFM4 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018).
A specific band was detected for Olfactomedin-4/OLFM4 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olfactomedin-4/OLFM4

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF-stimulated clone-1), GW112 or hOlfD, is a 64 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of proteins. Mature Olfactomedin-4 is a 490 amino acid (aa) protein that is secreted mainly as multimers. Human Olfactomedin-4 is mainly expressed by gastrointestinal stem cells, developing granulocytes, type M4 acute myelogenous leukemias, and intestinal and prostate cancer cells and is upregulated by NF-kB signaling. It is reported to facilitate cell-cell adhesion by binding to lectins and cadherins, and to be anti-apoptotic. Mature human Olfactomedin-4 shares 71% aa sequence identity with mouse and rat Olfactomedin-4.

Entrez Gene IDs
10562 (Human); 380924 (Mouse); 290409 (Rat)
Alternate Names
Antiapoptotic protein GW112; bA209J19.1; GC1; G-CSF-stimulated clone 1 protein; GW112; GW112hOLfD; hGC-1; KIAA4294; olfactomedin 4; Olfactomedin-4; OlfD; OLFM4; OLM4; UNQ362

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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