Human Olfactomedin-4/OLFM4 Antibody

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Detection of Human Olfactomedin-4/OLFM4 by Western Blot.
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Human Olfactomedin-4/OLFM4 Antibody Summary

Species Reactivity
Detects human Olfactomedin-4/OLFM4 in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 806305
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Olfactomedin-4/OLFM4
Accession # Q6UX06
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Olfactomedin-4/OLFM4 antibody by Western Blot. View Larger

Detection of Human Olfactomedin-4/OLFM4 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Olfactomedin-4/OLFM4 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olfactomedin-4/OLFM4

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF-stimulated clone-1), GW112 or hOlfD, is a 64 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of proteins. Mature Olfactomedin-4 is a 490 amino acid (aa) protein that is secreted mainly as multimers. Human Olfactomedin-4 is mainly expressed by gastrointestinal stem cells, developing granulocytes, type M4 acute myelogenous leukemias, and intestinal and prostate cancer cells and is upregulated by NF-kB signaling. It is reported to facilitate cell-cell adhesion by binding to lectins and cadherins, and to be anti-apoptotic. Mature human Olfactomedin-4 shares 71% aa sequence identity with mouse and rat Olfactomedin-4.

Entrez Gene IDs
10562 (Human); 380924 (Mouse); 290409 (Rat)
Alternate Names
Antiapoptotic protein GW112; bA209J19.1; GC1; G-CSF-stimulated clone 1 protein; GW112; GW112hOLfD; hGC-1; KIAA4294; olfactomedin 4; Olfactomedin-4; OlfD; OLFM4; OLM4; UNQ362

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Human Olfactomedin-4/OLFM4 Antibody
By Anonymous on 09/22/2021
Application: Western Blot Sample Tested: SW480 human colorectal adenocarcinoma cell line Species: Human