Human Ornithine Carbamoyltransferase/OTC Antibody

Catalog # Availability Size / Price Qty
MAB9918-100
MAB9918-SP

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Ornithine Carbamoyltransferase/OTC in Human Liver.
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Human Ornithine Carbamoyltransferase/OTC Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ornithine Carbamoyltransferase/OTC in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 990421
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human Ornithine Carbamoyltransferase/OTC
Accession # P00480
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Ornithine Carbamoyltransferase/OTC in Human Liver. View Larger

Ornithine Carbamoyltransferase/OTC in Human Liver. Ornithine Carbamoyltransferase/OTC was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Ornithine Carbamoyltransferase/OTC Monoclonal Antibody (Catalog # MAB9918) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ornithine Carbamoyltransferase/OTC

Human Ornithine transcarbamylase (OTC) is a 354 amino acids, 40 KDa enzyme that catalyzes the reaction between carbamoyl phosphate (CP) and ornithine (Orn) to form citrulline (Cit) and phosphate (Pi). OTC is a trimer. The monomer unit has a CP-binding domain and an amino acid-binding domain. The active sites are located at the interface between the protein monomers.

Entrez Gene IDs
5009 (Human); 18416 (Mouse); 25611 (Rat)
Alternate Names
EC 2.1.3; EC 2.1.3.3; MGC129967; MGC129968; MGC138856; OCTD; Ornithine Carbamoyltransferase; ornithine carbamoyltransferase, mitochondrial; Ornithine Transcarbamylase; OTC; OTCase

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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