Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PAM Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PAM
Phe21-Val817
Accession # P19021

Specificity

Detects human PAM in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human PAM Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human PAM

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PAM

PAM (Peptidyl-Gly alpha -amidating monooxygenase) is a dual-function, 95‑110 kDa protein that belongs to both the lyase and monooxygenase enzyme families. It is expressed in a variety of endocrine and exocrine glands and catalyzes the conversion of C‑terminal Gly into amidated residues. Human ProPAM is a 953 amino acid (aa) type I transmembrane glycoprotein. It contains an 833 aa extracellular domain (ECD) (aa 31‑863) and an 86 aa cytoplasmic tail. The ECD shows one PHM/monooxygenase region (aa 40‑347) and a PAL/lyase domain (aa 495‑817). Both regions are enzymatically active. Alternative splicing may generate a soluble form (aa 31‑828), and remove aa 388‑494 that link the two embedded enzyme segments. Over aa 21‑817, human PAM is 91% aa identical to mouse PAM.

Long Name

Peptidylglycine Alpha-Amidating Monooxygenase

Alternate Names

PAL, PHM

Entrez Gene IDs

5066 (Human); 18484 (Mouse); 25508 (Rat)

Gene Symbol

PAM

UniProt

P19021

Additional PAM Products

Product Documents for Human PAM Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PAM Antibody

For research use only

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

Citations for Human PAM Antibody

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