Human PAM Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Human PAM Antibody Summary

Species Reactivity
Detects human PAM in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human PAM
Accession # P19021
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human PAM

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAM

PAM (Peptidyl-Gly alpha -amidating monooxygenase) is a dual-function, 95‑110 kDa protein that belongs to both the lyase and monooxygenase enzyme families. It is expressed in a variety of endocrine and exocrine glands and catalyzes the conversion of C‑terminal Gly into amidated residues. Human ProPAM is a 953 amino acid (aa) type I transmembrane glycoprotein. It contains an 833 aa extracellular domain (ECD) (aa 31‑863) and an 86 aa cytoplasmic tail. The ECD shows one PHM/monooxygenase region (aa 40‑347) and a PAL/lyase domain (aa 495‑817). Both regions are enzymatically active. Alternative splicing may generate a soluble form (aa 31‑828), and remove aa 388‑494 that link the two embedded enzyme segments. Over aa 21‑817, human PAM is 91% aa identical to mouse PAM.

Long Name
Peptidylglycine Alpha-Amidating Monooxygenase
Entrez Gene IDs
5066 (Human); 18484 (Mouse); 25508 (Rat)
Alternate Names
PAL; PAM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylamidoglycolate lyase; peptidylglycine 2-hydroxylase; peptidyl-glycine alpha-amidating monooxygenase; peptidylglycine alpha-amidating monooxygenase; peptidylglycine alpha-hydroxylating monooxygenase; PHM

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Citation for Human PAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. PAM staining intensity of primary neuroendocrine neoplasms is a potential prognostic biomarker
    Authors: TM Horton, V Sundaram, CH Lee, K Hornbacker, A Van Vleck, KN Benjamin, A Zemek, TA Longacre, PL Kunz, JP Annes
    Sci Rep, 2020;10(1):10943.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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