Human PD-ECGF/Thymidine Phosphorylase Antibody

Catalog # Availability Size / Price Qty
AB-229-NA
Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase  and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody.
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Human PD-ECGF/Thymidine Phosphorylase Antibody Summary

Species Reactivity
Human
Specificity
Detects human PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with ____________ is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD-ECGF/Thymidine Phosphorylase
Ala11-Gln482
Accession # P19971.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PD-ECGF (Catalog # 229-PE)
Neutralization
Measured by its ability to neutralize PD‑ECGF-induced proliferation in HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The Neutralization Dose (ND50) is typically 10-25 µg/mL in the presence of 150 ng/mL Recombinant Human PD‑ECGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase  and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody. View Larger

Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase  and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody.

Recombinant Human PD‑ECGF/Thymidine Phosphorylase (Catalog # 229‑PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human  PD‑ECGF/Thymidine Phosphorylase (150 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Human  PD‑ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND50 is typically 10‑25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-ECGF/Thymidine Phosphorylase

PD-ECGF was originally isolated as a platelet-derived endothelial cell mitogen (based on its ability to stimulate 3H-thymidine incorporation) and its cDNA was cloned from a human placental cDNA library. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The sequence of human PD-ECGF is identical to that of human thymidine phosphorylase (dThdPase).

References
  1. Haraguchi, M. et al. (1994) Nature 368:198.
  2. Toi, M. et al. (2005) Lancet Oncol. 6:158.
  3. Akiyama, S. et al. (2004) Canc. Sci. 95:851.
Long Name
Platelet-derived Endothelial Cell Growth Factor
Entrez Gene IDs
1890 (Human); 72962 (Mouse)
Alternate Names
dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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