Human PD-ECGF/Thymidine Phosphorylase Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with ____________ is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD-ECGF/Thymidine Phosphorylase
    Ala11-Gln482
    Accession # P19971.2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human PD-ECGF (Catalog # 229-PE)
  • Neutralization
    Measured by its ability to neutralize PD‑ECGF-induced proliferation in HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The Neutralization Dose (ND50) is typically 10-25 µg/mL in the presence of 150 ng/mL Recombinant Human PD‑ECGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase  and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody.

Recombinant Human PD‑ECGF/Thymidine Phosphorylase (Catalog # 229‑PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human  PD‑ECGF/Thymidine Phosphorylase (150 ng/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Human  PD‑ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND50 is typically 10‑25 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-ECGF/Thymidine Phosphorylase
PD-ECGF was originally isolated as a platelet-derived endothelial cell mitogen (based on its ability to stimulate 3H-thymidine incorporation) and its cDNA was cloned from a human placental cDNA library. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The sequence of human PD-ECGF is identical to that of human thymidine phosphorylase (dThdPase).
  • References:
    1. Haraguchi, M. et al. (1994) Nature 368:198.
    2. Toi, M. et al. (2005) Lancet Oncol. 6:158.
    3. Akiyama, S. et al. (2004) Canc. Sci. 95:851.
  • Long Name:
    Platelet-derived Endothelial Cell Growth Factor
  • Entrez Gene IDs:
    1890 (Human); 72962 (Mouse)
  • Alternate Names:
    dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP
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