Measured by its ability to neutralize PD‑ECGF-induced proliferation in HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The Neutralization Dose (ND50) is typically 10-25 µg/mL in the presence of 150 ng/mL Recombinant Human PD‑ECGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody.
Recombinant Human PD‑ECGF/Thymidine Phosphorylase (Catalog # 229‑PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD‑ECGF/Thymidine Phosphorylase (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PD‑ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND50 is typically 10‑25 µg/mL.
Preparation and Storage
Reconstitute at 1 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-ECGF/Thymidine Phosphorylase
PD-ECGF was originally isolated as a platelet-derived endothelial cell mitogen (based on its ability to stimulate 3H-thymidine incorporation) and its cDNA was cloned from a human placental cDNA library. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The sequence of human PD-ECGF is identical to that of human thymidine phosphorylase (dThdPase).
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.