Human PD-L1 DuoSet ELISA

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Human PD-L1 / B7-H1 ELISA Standard Curve
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Product Details
Citations (9)
Supplemental Products
Reviews (1)

Human PD-L1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
156.3 - 10,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PD-L1 / B7-H1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (R&D Systems®, Catalog # DY005).

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human PD-L1 / B7-H1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Long Name:
Programmed Death Ligand 1
Entrez Gene IDs:
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names:
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Citations for Human PD-L1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients
    Authors: Miladinov, M;Rosic, J;Eric, K;Guzonjic, A;Jelenkovic, J;Bogavac-Stanojevic, N;Dimitrijevic, I;Kotur-Stevuljevic, J;Barisic, G;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  2. Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood
    Authors: K Krueger, Z Mayer, M Kottmaier, M Gerckens, S Boeck, P Luppa, S Holdenried
    Biomedicines, 2022-10-11;10(10):.
    Species: Human
    Sample Types: Plasma
  3. High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood
    Authors: K Krueger, Z Mayer, M Gerckens, S Boeck, P Luppa, S Holdenried
    Biomedicines, 2022-09-26;10(10):.
    Species: Human
    Sample Types: Plasma
  4. Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis
    Authors: M Roksandic, A Klisic, V Ceriman, J Kotur Stev, K Savic Vujo, D Mirkov, M Gajic, B Ilic, N Dimic, N Samardzic, D Jovanovic
    European review for medical and pharmacological sciences, 2022-02-01;26(3):927-934.
    Species: Human
    Sample Types: Plasma
  5. Soluble PD-L1 Is an Independent Prognostic Factor in Clear Cell Renal Cell Carcinoma
    Authors: G Larrinaga, JD Solano-Itu, P Errarte, M Unda, A Loizaga-Ir, A Pérez-Fern, E Echevarría, A Asumendi, C Manini, JC Angulo, JI López
    Cancers, 2021-02-07;13(4):.
    Species: Human
    Sample Types: Plasma
  6. Are the Immune Properties of Mesenchymal Stem Cells from Wharton's Jelly Maintained during Chondrogenic Differentiation?
    Authors: C Voisin, G Cauchois, L Reppel, C Laroye, L Louarn, C Schenowitz, P Sonon, I Poras, V Wang, E D Carosell, N Benkirane-, P Moreau, N Rouas-Frei, D Bensoussan, C Huselstein
    J Clin Med, 2020-02-04;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study.
    Authors: Jovanovic D, Roksandic Milenkovic M, Kotur Stevuljevic J, Markovic J, Ceriman V, Kontic M, Skodric Trifunovic V
    J Thorac Dis, 2018-12-01;10(12):6660-6669.
    Species: Human
    Sample Types: Plasma
  8. Serum levels of soluble programmed death protein 1 (sPD-1) and soluble programmed death ligand 1 (sPD-L1) in advanced pancreatic cancer
    Authors: S Kruger, ML Legenstein, V Rösgen, M Haas, DP Modest, CB Westphalen, S Ormanns, T Kirchner, V Heinemann, S Holdenried, S Boeck
    Oncoimmunology, 2017-03-31;6(5):e1310358.
    Species: Human
    Sample Types: Serum
  9. Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression
    Stem Cells, 2016-10-26;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates


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Human PD-L1/B7-H1 DuoSet ELISA
By Leslie Priddy on 04/04/2018
Sample Tested: Serum