Human PD-L1 DuoSet ELISA
Human PD-L1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
Normal Goat Serum: (R&D Systems®, Catalog # DY005).
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: PD-L1/B7-H1
PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.
Citations for Human PD-L1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood
Authors: K Krueger, Z Mayer, M Kottmaier, M Gerckens, S Boeck, P Luppa, S Holdenried
Biomedicines, 2022;10(10):.
Species: Human
Sample Types: Plasma
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Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis
Authors: M Roksandic, A Klisic, V Ceriman, J Kotur Stev, K Savic Vujo, D Mirkov, M Gajic, B Ilic, N Dimic, N Samardzic, D Jovanovic
European review for medical and pharmacological sciences, 2022;26(3):927-934.
Species: Human
Sample Types: Plasma
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High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood
Authors: K Krueger, Z Mayer, M Gerckens, S Boeck, P Luppa, S Holdenried
Biomedicines, 2022;10(10):.
Species: Human
Sample Types: Plasma
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Soluble PD-L1 Is an Independent Prognostic Factor in Clear Cell Renal Cell Carcinoma
Authors: G Larrinaga, JD Solano-Itu, P Errarte, M Unda, A Loizaga-Ir, A Pérez-Fern, E Echevarría, A Asumendi, C Manini, JC Angulo, JI López
Cancers, 2021;13(4):.
Species: Human
Sample Types: Plasma
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Are the Immune Properties of Mesenchymal Stem Cells from Wharton's Jelly Maintained during Chondrogenic Differentiation?
Authors: C Voisin, G Cauchois, L Reppel, C Laroye, L Louarn, C Schenowitz, P Sonon, I Poras, V Wang, E D Carosell, N Benkirane-, P Moreau, N Rouas-Frei, D Bensoussan, C Huselstein
J Clin Med, 2020;9(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Serum levels of soluble programmed death protein 1 (sPD-1) and soluble programmed death ligand 1 (sPD-L1) in advanced pancreatic cancer
Authors: S Kruger, ML Legenstein, V Rösgen, M Haas, DP Modest, CB Westphalen, S Ormanns, T Kirchner, V Heinemann, S Holdenried, S Boeck
Oncoimmunology, 2017;6(5):e1310358.
Species: Human
Sample Types: Serum
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Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression
Stem Cells, 2016;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study.
Authors: Jovanovic D, Roksandic Milenkovic M, Kotur Stevuljevic J, Markovic J, Ceriman V, Kontic M, Skodric Trifunovic V
J Thorac Dis, 0;10(12):6660-6669.
Species: Human
Sample Types: Plasma
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