Human PDGF-AA DuoSet ELISA

  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Sample Type & Volume Required
    100 µL
  • Range
    15.60 - 1,000 pg/mL
  • Sufficient Materials
    For fifteen 96-well plates*
  • Specificity
    Please see the product datasheet
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.


Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
Kit Content
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AA
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
  • Entrez Gene IDs:
    5154 (Human); 25266 (Rat);
  • Long Name:
    Platelet-derived Growth Factor AA
DuoSet Ancillary Reagent Kits
Description Application Cat# Citations Images  

DuoSet ELISA Ancillary Reagent Kit 2

ELISA DY008
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)

ELISA DY999 38
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)

ELISA DY995 12
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Stop Solution 2N Sulfuric Acid (15 x 6 mL)

ELISA DY994 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

ELISA Plate Sealers, 100 Pack

ELISA DY992
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Quantikine Wash Buffer 1

ELISA WA126 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-HRP (set of 5 vials enough for 75 plates total)

ELISA DY998 29
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Clear Polystyrene Microplates, 25 Pack

ELISA DY990 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Additive 1 (21 mL)

ELISA DY005
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Glo Substrate Reagent Pack (Reagent A & B)

ELISA DY993 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

ELISA Plate-coating Buffer (250 mL of PBS)

ELISA DY006 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 3 (5X, 10 x 21 mL)

ELISA DY004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Black Polystyrene Microplates, 25 Pack

ELISA DY991 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human PDGF-AA DuoSet ELISA?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Feedback

Customer Information