Human PDZD2 Antibody

Catalog # Availability Size / Price Qty
MAB7670
MAB7670-SP
Detection of Human PDZD2 by Western Blot.
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Human PDZD2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDZD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse PDZD2 is observed.
Source
Monoclonal Mouse IgG2A Clone # 787924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PDZD2
Ser2489-Ser2839
Accession # O15018
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human PDZD2 antibody by Western Blot. View Larger

Detection of Human PDZD2 by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PDZD2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PDZD2 antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

PDZD2 in PC‑3 Human Cell Line. PDZD2 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDZD2

PDZD2 (PSD95, Discs-large and ZO-1 domain-containing protein 2), also called AIPC (activated in prostate cancer) and PDZK3, is a multi-PDZ-domain protein that is ubiquitously expressed. The 2839 amino acid (aa) human PDZD2 is found in the endoplasmic reticulum. Caspase cleavage produces the 347 aa, 37 kDa secreted mature form (aa 2493-2839). A 2640 aa isoform is lacking 199 aa N-terminal to the mature sequence. The processed, mature form contains two PDZ domains which interact with the C-terminus of partner proteins, promotes maturation of pancreatic b cells, and is highly expressed in human prostate tumors. Human PDZD2 shares 72% aa sequence identity with mouse or rat PDZD2.

Long Name
PDZ Domain Containing 2
Entrez Gene IDs
23037 (Human); 68070 (Mouse); 65034 (Rat)
Alternate Names
Activated in prostate cancer protein; AIPC; AIPCPAPIN; KIAA0300; KIAA0300PDZ domain-containing protein 3; Papin; PDZ domain containing 2; PDZ domain containing 3; PDZ domain-containing protein 2; PDZD2; PDZK3; Pin1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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