Detection of Human PDZD2 by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PDZD2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDZD2 in PC‑3 Human Cell Line. PDZD2 was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(PSD95, Discs-large and ZO-1 domain-containing protein 2), also called AIPC (activated
in prostate cancer) and PDZK3, is a multi-PDZ-domain protein that is
ubiquitously expressed. The 2839 amino acid (aa) human PDZD2 is found in the
endoplasmic reticulum. Caspase cleavage produces the 347 aa, 37 kDa secreted
mature form (aa 2493-2839). A 2640 aa isoform is lacking 199 aa N-terminal to
the mature sequence. The processed, mature form contains two PDZ domains which
interact with the C-terminus of partner proteins, promotes maturation of
cells, and is highly expressed in human prostate tumors. Human PDZD2 shares 72%
aa sequence identity with mouse or rat PDZD2.
PDZ Domain Containing 2
Entrez Gene IDs:
23037 (Human); 68070 (Mouse); 65034 (Rat)
Activated in prostate cancer protein; AIPC; AIPCPAPIN; KIAA0300; KIAA0300PDZ domain-containing protein 3; Papin; PDZ domain containing 2; PDZ domain containing 3; PDZ domain-containing protein 2; PDZD2; PDZK3; Pin1
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