Human PDZD2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PDZD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse PDZD2 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 787924
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PDZD2
    Ser2489-Ser2839
    Accession # O15018
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PDZD2 by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PDZD2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
PDZD2 in PC‑3 Human Cell Line. PDZD2 was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDZD2
PDZD2 (PSD95, Discs-large and ZO-1 domain-containing protein 2), also called AIPC (activated in prostate cancer) and PDZK3, is a multi-PDZ-domain protein that is ubiquitously expressed. The 2839 amino acid (aa) human PDZD2 is found in the endoplasmic reticulum. Caspase cleavage produces the 347 aa, 37 kDa secreted mature form (aa 2493-2839). A 2640 aa isoform is lacking 199 aa N-terminal to the mature sequence. The processed, mature form contains two PDZ domains which interact with the C-terminus of partner proteins, promotes maturation of pancreatic b cells, and is highly expressed in human prostate tumors. Human PDZD2 shares 72% aa sequence identity with mouse or rat PDZD2.
  • Long Name:
    PDZ Domain Containing 2
  • Entrez Gene IDs:
    23037 (Human); 68070 (Mouse); 65034 (Rat)
  • Alternate Names:
    Activated in prostate cancer protein; AIPC; AIPCPAPIN; KIAA0300; KIAA0300PDZ domain-containing protein 3; Papin; PDZ domain containing 2; PDZ domain containing 3; PDZ domain-containing protein 2; PDZD2; PDZK3; Pin1
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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2A PE-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2A APC-conjugated Antibody

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Mouse IgG2A Antibody

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

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