Human Peroxiredoxin 6 (Prx-6) is a 26 kDa, cytosolic antioxidant enzyme that belongs to the 1-Cys class of the THP/ahpC family of proteins. This protein is 224 amino acids (aa) in length and has one catalytic cysteine at Cys46. Following an attack on peroxide, Cys46 is oxidized to cysteine sulfenic acid and exists in a stable conformation. Reduced, Prx-6 is a homotetramer. When activated, it apparently forms a covalently-associated homodimer. In addition to glutathione peroxidase activity, Prx-6 is also reported to demonstrate phospholipase A2 activity. Thus, Prx-6 likely plays a role in phospholipid turnover. Ser32 in a GWSWG motif is required for its enzymatic activity. Human Prx-6 is 92% and 90% aa identical to rat and mouse Prx-6, respectively.
Human Peroxiredoxin 6 Antibody
R&D Systems | Catalog # MAB3490R
Key Product Details
Validated by
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met1-Pro224
Accession # P30041
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Peroxiredoxin 6 Antibody
Detection of Human Peroxiredoxin 6 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Peroxiredoxin 6 Monoclonal Antibody (Catalog # MAB3490R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Peroxiredoxin 6 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Peroxiredoxin 6 Specificity Using Knockout Cell Line.
Western blot shows lysates of HAP1 human near-haploid cell line and Peroxiredoxin 6 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse Anti-Human Peroxiredoxin 6 Monoclonal Antibody (Catalog # MAB3490R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 6 at approximately 25 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.Applications for Human Peroxiredoxin 6 Antibody
Knockout Validated
Western Blot
Sample: HEK293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Peroxiredoxin 6
Alternate Names
Gene Symbol
UniProt
Additional Peroxiredoxin 6 Products
Product Documents for Human Peroxiredoxin 6 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Peroxiredoxin 6 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars