Human Phospho-BRCA1 (S1423) Antibody

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  • Species Reactivity
    Human
  • Specificity
    Detects human Phospho-BRCA1 when phosphorylated at S1423 in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human BRCA1 S1423 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed U2OS human osteosarcoma cell line treated with camphtothecin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human Phospho-BRCA1 (S1423) by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line mock treated or exposed to 10 Gy IR for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-BRCA1 (S1423) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1386) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-BRCA1 (S1423) was detected at approximately 220 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BRCA1

The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is phosphorylated at S1423 by the ATM (Ataxia‑Telangiectasia Mutated) and ATR (ATM-and Rad3-related) protein kinases in response to genotoxic stress.

  • Long Name:
    Breast Cancer 1
  • Entrez Gene IDs:
    672 (Human); 12189 (Mouse); 497672 (Rat)
  • Alternate Names:
    BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1
Related Research Areas

FAQs

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Phospho-BRCA1 (S1423) Antibody AF1386 Array AF1386
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Excellent
 WB Human Anonymous 01/17/2018
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Image Details
Western Blot Human Phospho-BRCA1 (S1423) Antibody AF1386
Western Blot: Human Phospho-BRCA1 (S1423) Antibody [AF1386].

Summary

Application Western Blot
Sample Tested MDA-MBA-231 human breast cancer cell line
Species Human

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