The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is phosphorylated at S1423 by the ATM (Ataxia‑Telangiectasia Mutated) and ATR (ATM-and Rad3-related) protein kinases in response to genotoxic stress.
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Phosphopeptide containing human BRCA1 S1423 site
Specificity
Detects human Phospho-BRCA1 when phosphorylated at S1423 in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human phospho-BRCA1 (S1423) Antibody
Detection of Human Phospho-BRCA1 (S1423) by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line mock treated or exposed to 10 Gy IR for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-BRCA1 (S1423) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1386) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-BRCA1 (S1423) was detected at approximately 220 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human phospho-BRCA1 (S1423) Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line treated with camphtothecin
Sample: Immersion fixed U2OS human osteosarcoma cell line treated with camphtothecin
Western Blot
0.5 µg/mL
Sample: U2OS human osteosarcoma cell line exposed to 10 Gy IR
Sample: U2OS human osteosarcoma cell line exposed to 10 Gy IR
Reviewed Applications
Read 1 review rated 5 using AF1386 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: BRCA1
Long Name
Breast Cancer 1
Alternate Names
BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1
Gene Symbol
BRCA1
Additional BRCA1 Products
Product Documents for Human phospho-BRCA1 (S1423) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human phospho-BRCA1 (S1423) Antibody
For research use only
Related Research Areas
Customer Reviews for Human phospho-BRCA1 (S1423) Antibody (1)
5 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: MDA-MBA-231 human breast cancer cell lineSpecies: HumanVerified Customer | Posted 01/17/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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