Human Phospho-BRCA1 (S1423) Antibody

Catalog # Availability Size / Price Qty
Human Phospho-BRCA1 (S1423) Antibody in Western Blot
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Product Details
Supplemental Products

Human Phospho-BRCA1 (S1423) Antibody Summary

Species Reactivity
Detects human Phospho-BRCA1 when phosphorylated at S1423 in Western blots.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing human BRCA1 S1423 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
5-15 µg/mL
Immersion fixed U2OS human osteosarcoma cell line treated with camphtothecin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-BRCA1 (S1423) by Western Blot. View Larger

Detection of Human Phospho-BRCA1 (S1423) by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line mock treated or exposed to 10 Gy IR for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-BRCA1 (S1423) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1386) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-BRCA1 (S1423) was detected at approximately 220 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U ?-phosphatase (?-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRCA1

The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is phosphorylated at S1423 by the ATM (Ataxia‑Telangiectasia Mutated) and ATR (ATM-and Rad3-related) protein kinases in response to genotoxic stress.

Long Name
Breast Cancer 1
Entrez Gene IDs
672 (Human); 12189 (Mouse); 497672 (Rat)
Alternate Names
BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1

Product Datasheets


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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control


Reconstitution Buffers

Secondary Antibodies

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