Human Phospho-BRCA1 (S1423) Antibody

Catalog # Availability Size / Price Qty
AF1386
AF1386-SP
Detection of Human Phospho-BRCA1 (S1423) by Western Blot.
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Human Phospho-BRCA1 (S1423) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Phospho-BRCA1 when phosphorylated at S1423 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human BRCA1 S1423 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed U2OS human osteosarcoma cell line treated with camphtothecin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-BRCA1 (S1423) by Western Blot. View Larger

Detection of Human Phospho-BRCA1 (S1423) by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line mock treated or exposed to 10 Gy IR for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-BRCA1 (S1423) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1386) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-BRCA1 (S1423) was detected at approximately 220 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRCA1

The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is phosphorylated at S1423 by the ATM (Ataxia‑Telangiectasia Mutated) and ATR (ATM-and Rad3-related) protein kinases in response to genotoxic stress.

Long Name
Breast Cancer 1
Entrez Gene IDs
672 (Human); 12189 (Mouse); 497672 (Rat)
Alternate Names
BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-BRCA1 (S1423) Antibody

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Human Phospho-BRCA1 (S1423) Antibody
By Anonymous on 01/17/2018
Application: WB Sample Tested: MDA-MBA-231 human breast cancer cell line Species: Human