Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2734A
View all CROCC Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human CROCC S site 1476
Accession # Q5TZA2

Specificity

Detects Human Phospho-CROCC in direct ELISA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-CROCC (S1476) Antibody

Detection of Human CROCC by Western Blot.

Western blot shows lysates of MDA‑MB‑231 human breast cancer cells and MDA-MB-231 + Calyculin A. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CROCC Monoclonal Antibody (Catalog # MAB11335) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CROCC at approximately ~228 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CROCC in HEL 92.1.7 and Daudi cells (both positive).

CROCC was detected in immersion fixed HEL 92.1.7 Human Erythroleukemic Cells and Daudi Human Burkitt's Lymphoma Cells (Both Positive) using Rabbit Anti-Human CROCC Monoclonal Antibody (Catalog # MAB11335) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Phospho-CROCC (S1476) Antibody

Application
Recommended Usage

Immunocytochemistry

3-15 µg/mL
Sample: Immersion fixed HEL 92.1.7 Human Erythroleukemic Cells and Daudi Human Burkitt's Lymphoma Cells (Both Positive)

Western Blot

1 µg/mL
Sample: MDA‑MB‑231 human breast cancer cells and MDA-MB-231 + Calyculin A

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CROCC

Human CROCC (also known as Rootletin) is a large coiled-coil protein that is primarily expressed in ciliated cells and plays a critical role in the organization and maintenance of the ciliary structure. Cilia are hair-like structures that protrude from the surface of many eukaryotic cells and are involved in a variety of cellular functions, including motility and signaling.CROCC is essential for forming and maintaining the rootlet and has been shown to interact with other centrosomal and ciliary proteins, such as PCM-1, C-Nap1, and CEP68. CROCC has also been shown to interact with microtubules, suggesting that it may play a role in regulating microtubule dynamics. CROCC can be Phosphorylated by NEK2 which may regulate its association with centrosomes. This antibody detects CROCC phosphorylated at Ser1476. Two alternatively spliced isoforms have been reported.

Long Name

Ciliary Rootlet Coiled-Coil, Rootletin

Alternate Names

CROCC1, KIAA0445, ROLT, TAX1BP2

Entrez Gene IDs

9696 (Human)

Gene Symbol

CROCC

UniProt

Q5TZA2

Additional CROCC Products

Product Documents for Human Phospho-CROCC (S1476) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Phospho-CROCC (S1476) Antibody

For research use only

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Protocols

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