Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Cercopithecus aethiops (African Green Monkey)

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1119D
View all DDR1/DDR2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human DDR2 Y740 site
Accession # Q16832

Specificity

Detects human DDR1 and DDR2 when phosphorylated at Y796 and Y740.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody

Detection of Human Phospho-DDR2 (Y740) antibody by Western Blot.

Detection of Human Phospho-DDR2 (Y740) by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DDR2 (Y740) at approximately 120-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-DDR2 (Y740) antibody by Simple WesternTM.

Detection of Human Phospho-DDR2 (Y740) by Simple WesternTM.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-DDR2 (Y740) at approximately 130 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Monoclonal Antibody (Catalog # MAB25382). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Prognostic value of DDR2 protein expression in HCC and its correlation with phosphorylated DDR2 (p-DDR2) in HCC cells. a Overall survival for DDR2 protein expression (log-rank, P < 0.01). b Disease-free survival for DDR2 protein expression (log-rank, P < 0.01). c Immunoblotting analysis of DDR2 and p-DDR2 in Hep3B cells with DDR2 expressing plasmid transfection or control plasmid transfection. d Immunoblotting analysis of DDR2 and p-DDR2 in MHCC-97H cells with DDR2-shRNA transfection or control-shRNA transfection Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26362312), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

PAPP-A promotes DDR2 activation through collagen mRNA stabilization and IGF signaling in vitro. j Immunoblot of MCF-7 cells treated in vitro with culture media from MCF-7 (CTL), MCF-7PAPP-A (PA), or MCF-7PAPP-A/E483Q (E483Q) cells for 24 h. k Immunoblot of DDR2 and phospho-DDR2 in MCF-7 and MCF-7PAPP-A cells treated in vitro with recombinant IGF-1 at 10 nM for indicated time points Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31046834), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Loss of DDR2 abolishes cell invasion, Snail expression, and tumor growth by PAPP-A. a Immunoblot of indicated markers in MCF-7PAPP-A CTL and MCF-7PAPP-A DDR2−/− cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31046834), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Loss of DDR2 abolishes cell invasion, Snail expression, and tumor growth by PAPP-A. h Immunoblot of indicated markers in MCF-7PAPP-A cells treated for 48 h at the indicated concentrations of PQ401 and imatinib. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31046834), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

The expression of DDR2 in cell lines and HCC tissues. a DDR2 mRNA expression in the immortalized normal human liver cell line L-02 and five hepatoma cell lines (MHCC-97H, HepG2, Huh-7, Hep3B and SMMC-7721) using qRT-PCR (n = 3, *P < 0.01). b DDR2 protein expression in the immortalized normal human liver cell line L-02 and five hepatoma cell lines (MHCC-97H, HepG2, Huh-7, Hep3B and SMMC-7721) using immunoblotting (n = 3, P < 0.01). c DDR2 mRNA expression in HCC tissues (T) and corresponding adjacent no tumorous tissues (N) using qRT-PCR (n = 112, P < 0.01). d The protein expression of DDR2 and phosphorylated DDR2 (p-DDR2) in HCC tissues (T) and corresponding adjacent no tumorous tissues (N) using immunoblotting (n = 112, P < 0.01, respectively) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26362312), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Detection of Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) by Western Blot

Prognostic value of DDR2 protein expression in HCC and its correlation with phosphorylated DDR2 (p-DDR2) in HCC cells. a Overall survival for DDR2 protein expression (log-rank, P < 0.01). b Disease-free survival for DDR2 protein expression (log-rank, P < 0.01). c Immunoblotting analysis of DDR2 and p-DDR2 in Hep3B cells with DDR2 expressing plasmid transfection or control plasmid transfection. d Immunoblotting analysis of DDR2 and p-DDR2 in MHCC-97H cells with DDR2-shRNA transfection or control-shRNA transfection Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26362312), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody

Application
Recommended Usage

Simple Western

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human DDR2 treated with Calyculin A

Western Blot

0.1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human DDR2 treated with Calyculin A

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DDR1/DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).

References

  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Karn, T. et al. (1993) Oncogene 8:3433.
  3. Vogel, W. et al. (1997) Mol. Cell 1:13.
  4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
  6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
  7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
  8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
  9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
  10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
  11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
  12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Long Name

Discoidin Domain Receptor 1/Discoidin Domain Receptor 2

Alternate Names

6030432F18;AI323681;CAK;CD167;CD167a;DDR;DDR1;Drd1;EDDR1;HGK2;MCK10;Mpk6;NEP;NTRK4;PTK3;PTK3A;PTK3D;RTK6;TRKE

UniProt

Q16832

Additional DDR1/DDR2 Products

Product Documents for Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody

Certificate of Analysis

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Product Specific Notices for Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody

For research use only

Citations for Human Phospho-DDR1/DDR2 (DDR1 Y796, DDR2 Y740) Antibody

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