Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1014F
View all eNOS Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human eNOS S1177 site
Accession # P29474

Specificity

Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-eNOS (S1177) Antibody

Detection of Human eNOS antibody by Western Blot.

Detection of Human eNOS by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for eNOS at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human eNOS by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with Pervanadate (PV), loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 141 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Applications for Human Phospho-eNOS (S1177) Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate

Western Blot

0.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Pervanadate

Reviewed Applications

Read 2 reviews rated 5 using MAB9028 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eNOS

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tonehttp://en.wikipedia.org/wiki/Vascular_resistance" title="Vascular resistance"> by inhibiting smooth muscle contraction and platelethttp://en.wikipedia.org/wiki/Platelet" title="Platelet"> aggregation. A constitutive calciumhttp://en.wikipedia.org/wiki/Calcium_in_biology" title="Calcium in biology"> dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatushttp://en.wikipedia.org/wiki/Golgi_apparatus" title="Golgi apparatus"> within cells.

Long Name

Endothelial Nitric Oxide Synthase

Alternate Names

NOS3

Entrez Gene IDs

4846 (Human); 18127 (Mouse)

Gene Symbol

NOS3

UniProt

P29474

Additional eNOS Products

Product Documents for Human Phospho-eNOS (S1177) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-eNOS (S1177) Antibody

For research use only

Citations for Human Phospho-eNOS (S1177) Antibody

Customer Reviews for Human Phospho-eNOS (S1177) Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
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3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human Phospho-eNOS (S1177) Antibody
    Name: Sophie Millar
    Application: Western Blot
    Sample Tested: Human aortic endothelial cells
    Species: Human
    Verified Customer | Posted 01/19/2019
    Human Phospho-eNOS (S1177) Antibody MAB9028
  • Human Phospho-eNOS (S1177) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue
    Species: Guinea Pig
    Verified Customer | Posted 09/08/2017
    Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW. Regular Bis Tris Criterion Gel electrophoresis from BioRad was run with MES buffer. Immobilion transfer membrane purchased from Millipore.
    Human Phospho-eNOS (S1177) Antibody MAB9028

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