Human Phospho-eNOS (S1177) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
  Detection  of Human eNOS by Western Blot.
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Product Details
Citations (1)
Supplemental Products
Reviews (2)

Human Phospho-eNOS (S1177) Antibody Summary

Species Reactivity
Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western blots.
Recombinant Monoclonal Rabbit IgG Clone # 1014F
Protein A or G purified from cell culture supernatant
Phosphopeptide containing the human eNOS S1177 site
Accession # P29474
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human eNOS by Western Blot. View Larger

Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 μM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for eNOS at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eNOS

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells.

Long Name
Endothelial Nitric Oxide Synthase
Entrez Gene IDs
4846 (Human); 18127 (Mouse)
Alternate Names
Constitutive NOS; EC; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII

Product Datasheets

Citation for Human Phospho-eNOS (S1177) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation
    Authors: Hai-Xin Qian
    Exp Ther Med, 2016;12(5):3181-3188.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB


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Human Phospho-eNOS (S1177) Antibody
By Anonymous on 01/19/2019
Application: WB Sample Tested: Human aortic endothelial cells Species: Human

Human Phospho-eNOS (S1177) Antibody
By Anonymous on 09/08/2017
Application: Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW. Sample Tested: Heart tissue Species: Guinea Pig

Regular Bis Tris Criterion Gel electrophoresis from BioRad was run with MES buffer. Immobilion transfer membrane purchased from Millipore.