Human Phospho-eNOS (S1177) Antibody

Recombinant Monoclonal Antibody.
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1014F
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing the human eNOS S1177 site
    Accession # P29474
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 μM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for eNOS at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eNOS

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells.

  • Long Name:
    Endothelial Nitric Oxide Synthase
  • Entrez Gene IDs:
    4846 (Human); 18127 (Mouse)
  • Alternate Names:
    Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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Average Rating: 5 (Based on 1 review)

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Read what people are saying who have used Human Phospho-eNOS (S1177) Antibody.

We have 1 review tested in 1 species: Guinea Pig.
We have 1 review tested in 1 application: Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW..

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Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW.
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Images Ratings Applications Species Reviewed By Date Details
Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW. Human Phospho-eNOS (S1177) Antibody MAB9028
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 Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW. Guinea Pig Anonymous 09/08/2017
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Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW. Human Phospho-eNOS (S1177) Antibody MAB9028
Used 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW.: Human Phospho-eNOS (S1177) Antibody [MAB9028]

Summary

ApplicationUsed 1/500 dilution for WB and obtained a very clean and clear individual band at the expected MW.
Sample TestedHeart tissue
SpeciesGuinea Pig

Other Experimental Details

Other Experimental DetailsRegular Bis Tris Criterion Gel electrophoresis from BioRad was run with MES buffer. Immobilion transfer membrane purchased from Millipore.

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