Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

R&D Systems | Catalog # DYC1770-2

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Sample Type

Cell Lysates

Reactivity

Human

Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Economical alternative to Western blot
View all IGF-I R/IGF1R ELISA Kits »

Product Summary for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human IGF-I R in cell lysates. An immobilized capture antibody specific for IGF-I R binds both phosphorylated and unphosphorylated IGF-I R. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Kit Contents for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I R/IGF1R

IGF-I R/IGF1R (Insulin-like Growth Factor I Receptor), also known as CD221, is a ubiquitously expressed heterotetrameric transmembrane glycoprotein consisting of two alpha and two beta subunits. It binds IGF-1 with high affinity, IGF2 with lower affinity, and Insulin with lowest affinity. IGF1R/Insulin R hybrids respond primarily to IGF-1 and may downregulate cellular responses to Insulin. IGF signaling is also modulated by IGF binding proteins and the scavenger receptor, IGF-II R/IGF2R. Mice lacking IGF1R show intrauterine growth deficiency and die at birth due to respiratory failure, and IGF1R mutations in humans can retarded pre- and postnatal growth. IGF-1 and its receptor are particularly important for neurogenesis, with deficiency producing microcephaly and learning disorders. IGF1R expression on human embryonic stem cells is important for their survival and clonogenicity.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

Additional IGF-I R/IGF1R Products

Product Documents for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

For research use only

Citations for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

Customer Reviews for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA (2)

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  • Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA
    Name: Patricia Carulla Martí
    Sample Tested: Cell lysate from Human Keratinocytes (Hacat) in presence or absence of IGF and Cultured Human Keratinocytes
    Verified Customer | Posted 06/22/2023
    Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA controls
    Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA DYC1770-2
  • Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 02/25/2023

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FAQs for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

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    • A: This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
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