Detection of Human Phospho-LAT (Y161) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-LAT (Y161) Monoclonal Antibody (Catalog # MAB6334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-LAT (Y161) at approximately 40 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Sheep Anti-Human LAT Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF6208). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LAT (Linker for Activation of T cells) is a type III transmembrane lipid raft protein that serves as a scaffold for signaling molecules. Isoforms of 262 (36 kDa) and 233, called long and short, respectively, differ by inclusion/exclusion of aa 114‑142; a 269 aa isoform lacks this region, but includes alternate N-terminal sequence. Upon T-cell antigen receptor activation, LAT is multiply phosphorylated by ZAP-70/Syk protein tyrosine kinases, creating docking sites for SH2 domain-containing proteins. Phospholipase C-gamma docks at Y161, which is pY132 in the short form of LAT. Mutation of this site results in a Th2 autoimmune lymphoproliferative disorder in mice.
Linker for Activation of T cells
Entrez Gene IDs:
27040 (Human); 16797 (Mouse); 81511 (Rat)
36 kDa phospho-tyrosine adapter protein; LAT; LAT1,36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells; linker for activation of T-cells family member 1; p36-38; pp36
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