Human Phospho-MSP R/Ron (Y1238/Y1239) Antibody

Catalog # Availability Size / Price Qty
AF1947
AF1947-SP
Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot.
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Product Details
Citations (2)
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Human Phospho-MSP R/Ron (Y1238/Y1239) Antibody Summary

Species Reactivity
Human
Specificity
The antibody detects endogenous human MSP R/Ron phosphorylated at Y1238/Y1239 using Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
Phosphopeptide containing human MSP R/Ron Y1238/Y1239 sites
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot. View Larger

Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot. Western blot shows lysates of MDA‑MB‑453 human breast cancer cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase (CIP). PVDF membrane was probed with 1 µg/mL of Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection  of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western blot. View Larger

Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western blot. Human Phospho-MSP R/Ron (Y1238/Y1239) was immunoprecipitated from 1 mg of lysates of MDA‑MB‑453 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSP R/Ron

Macrophage stimulating protein receptor (MSP R), also known as Ron, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met and chicken Sea. Each family member is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha  chain and a membrane-spanning beta  chain with intrinsic tyrosine kinase activity. MSP R/Ron is overexpressed in a number of primary breast carcinomas and non-small cell lung carcinomas, suggesting a role for this RTK in mammary and lung tumor progression. Phosphorylation of MSP R/Ron at Y1238/Y1239 in the activation loop of the kinase domain is responsible for kinase activity.

Long Name
Macrophage Stimulating Protein Receptor
Entrez Gene IDs
4486 (Human); 19882 (Mouse)
Alternate Names
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

Product Datasheets

Citations for Human Phospho-MSP R/Ron (Y1238/Y1239) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-beta in gastric tissue.
    Authors: Ota M, Horiguchi M, Fang V, Shibahara K, Kadota K, Loomis C, Cammer M, Rifkin D
    Cancer Res, 2014;74(9):2642-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  2. The Ron receptor tyrosine kinase positively regulates angiogenic chemokine production in prostate cancer cells.
    Authors: Thobe MN, Gurusamy D, Pathrose P, Waltz SE
    Oncogene, 2010;29(2):214-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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