Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody

Catalog #: AF1947
7 Citations Datasheet / COA / SDS

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AF1947
AF1947-SP

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Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot.

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Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody Summary

Species Reactivity

Human

Specificity

The antibody detects endogenous human MSP R/Ron phosphorylated at Y1238/Y1239 using Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen and protein A Affinity-purified

Immunogen

Phosphopeptide containing human MSP R/Ron Y1238/Y1239 sites

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot. Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase (CIP). PVDF membrane was probed with 1 µg/mL of Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western blot. Human Phospho-MSP R/Ron (Y1238/Y1239) was immunoprecipitated from 1 mg of lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), also known as Ron, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met and chicken Sea. Each family member is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha chain and a membrane-spanning beta chain with intrinsic tyrosine kinase activity. MSP R/Ron is overexpressed in a number of primary breast carcinomas and non-small cell lung carcinomas, suggesting a role for this RTK in mammary and lung tumor progression. Phosphorylation of MSP R/Ron at Y1238/Y1239 in the activation loop of the kinase domain is responsible for kinase activity.

Long Name

Macrophage Stimulating Protein Receptor

Entrez Gene IDs

4486 (Human); 19882 (Mouse)

Alternate Names

CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
The Journal of Biological Chemistry, 2022;0(0):102029.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
Recepteur d'origine nantais contributes to the development of endometriosis via promoting epithelial-mesenchymal transition of a endometrial epithelial cells
Authors: Q Yu, J Wang, T Li, X Guo, S Ding, X Che, L Zhu, Y Peng, X Xu, G Zou, X Zhang
Journal of Cellular and Molecular Medicine, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Analysis of the Effect of Increased &alpha2,3-Sialylation on RTK Activation in MKN45 Gastric Cancer Spheroids Treated with Crizotinib
Authors: M Balmaña, F Diniz, T Feijão, CC Barrias, S Mereiter, CA Reis
Int J Mol Sci, 2020;21(3):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
RON signalling promotes therapeutic resistance in ESR1 mutant breast cancer
Authors: D Dustin, G Gu, AR Beyer, SK Herzog, DG Edwards, H Lin, TL Gonzalez, SL Grimm, C Coarfa, DW Chan, BJ Kim, JP De La O, MJ Ellis, D Liu, S Li, AL Welm, SAW Fuqua
British Journal of Cancer, 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer
Authors: R Graves-Dea, G Bogatcheva, S Rehman, Y Lu, JN Higginboth, B Singh
Oncotarget, 2019;10(13):1320-1333.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-beta in gastric tissue.
Authors: Ota M, Horiguchi M, Fang V, Shibahara K, Kadota K, Loomis C, Cammer M, Rifkin D
Cancer Res, 2014;74(9):2642-51.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
The Ron receptor tyrosine kinase positively regulates angiogenic chemokine production in prostate cancer cells.
Authors: Thobe MN, Gurusamy D, Pathrose P, Waltz SE
Oncogene, 2010;29(2):214-26.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot