Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human p27/Kip1 T157 site

Specificity

Detects human p27/Kip1 when phosphorylated at T157 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-p27/Kip1 (T157) Antibody

Detection of Human Phospho-p27/Kip1 (T157) antibody by Western Blot.

Detection of Human Phospho-p27/Kip1 (T157) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-p27/Kip1 (T157) Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: MCF-7 human breast cancer cell line treated with Recombinant Human IGF-I (Catalog # 291-G1)

Reviewed Applications

Read 1 review rated 4 using AF1555 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. The Akt protein kinase phosphorylates T157, an amino acid residue that lies within a nuclear localization signal for p27, to prevent nuclear localization and thus preclude the growth inhibitory function of p27.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Entrez Gene IDs

1027 (Human); 12576 (Mouse); 83571 (Rat)

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for Human Phospho-p27/Kip1 (T157) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Phospho-p27/Kip1 (T157) Antibody

For research use only

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

Citations for Human Phospho-p27/Kip1 (T157) Antibody

Customer Reviews for Human Phospho-p27/Kip1 (T157) Antibody (1)

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1 Customer Rating
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  • Human Phospho-p27/Kip1 (T157) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human breast cancer cell line SKBR3 and this product
    Species: Human
    Verified Customer | Posted 01/11/2017
    Immunoblot analysis of phospho p27-Thr157 in p27 immunoprecipitates from SKBr3 cells treated (+) or not (-) with alkaline phosphatase (AP). Primary Ab 1:1000 at 4°C overnight. Secondary Ab 1:20000 at 4°C 1h. Pvdf membrane.
    Human Phospho-p27/Kip1 (T157) Antibody AF1555

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