Human Phospho-p27/Kip1 (T157) Antibody

(11 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human p27/Kip1 when phosphorylated at T157 in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human p27/Kip1 T157 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-p27/Kip1 (T157) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291‑G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. The Akt protein kinase phosphorylates T157, an amino acid residue that lies within a nuclear localization signal for p27, to prevent nuclear localization and thus preclude the growth inhibitory function of p27.

  • Long Name:
    p27 Cyclin Dependent Kinase 4 Inhibitor 1B
  • Entrez Gene IDs:
    1027 (Human); 12576 (Mouse); 83571 (Rat)
  • Alternate Names:
    CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
    Authors: Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J
    Oncogene, 2015;34(43):5447-59.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  2. SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1.
    Authors: Wang Y, Zhou D, Chen S
    Mol Endocrinol, 2014;28(6):935-48.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. TRIP6 regulates p27 KIP1 to promote tumorigenesis.
    Authors: Lin, Victor T, Lin, Vivian Y, Lai, Yun-Ju, Chen, Chen-Sha, Liu, Kang, Lin, Weei-Chi, Lin, Fang-Tsy
    Mol Cell Biol, 2013;33(7):1394-409.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
    Authors: Wen S, So Y, Singh K
    Oncogene, 2012;31(14):1771-80.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt.
    Authors: Shiozaki A, Shen-Tu G, Bai X, Iitaka D, De Falco V, Santoro M, Keshavjee S, Liu M
    PLoS ONE, 2012;7(8):e43646.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
    Authors: Santi SA, Lee H
    PLoS ONE, 2011;6(1):e14614.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility.
    Authors: Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A
    J. Biol. Chem., 2011;286(33):29005-13.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Akt isoform-specific inhibition of MDA-MB-231 cell proliferation.
    Authors: Yang W, Ju JH, Lee KM, Shin I
    Cell. Signal., 2011;23(1):19-26.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery.
    Authors: Rodriguez-Ubreva FJ, Cariaga-Martinez AE, Cortes MA, Romero-De Pablos M, Ropero S, Lopez-Ruiz P, Colas B
    Oncogene, 2010;29(3):345-55.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  10. Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
    Authors: Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL
    Clin. Cancer Res., 2009;15(1):81-90.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  11. Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor.
    Authors: Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS
    Mol. Pharmacol., 2006;70(5):1534-41.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 11 Citations
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Rabbit IgG Antibody

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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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