Human Phospho-p53 (S15) Antibody

Catalog # Availability Size / Price Qty
AF1043
AF1043-SP
Detection of Human Phospho-p53 (S15) by Western Blot.
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Product Details
Citations (4)
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Human Phospho-p53 (S15) Antibody Summary

Species Reactivity
Human
Specificity
Detects human, monkey, and rat p53 when phosphorylated at S15 and mouse p53 when phosphorylated at S18.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human p53 S15 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
1 µg/500 µg cell lysate
CEM human T-lymphoblastoid cell line exposed to UV-C and MCF‑7 human breast cancer cell line treated with camptothecin (CPT), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-p53 (S15) by Western Blot. View Larger

Detection of Human Phospho-p53 (S15) by Western Blot. Western blot shows lysates of CEM human T-lymphoblastoid cell line untreated or exposed to 100 J/m2 UV-C for the indicated time. PVDF membrane was probed with 0.2 µg/mL Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-p53 (S15) in HeLa Human Cell Line. View Larger

Phospho-p53 (S15) in HeLa Human Cell Line. p53 phosphorylated at S15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with UV light (left panel) or untreated (right panel) using Rabbit Anti-Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using Rat Anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Phospho-p53 (S15) in Human Breast Cancer Tissue. View Larger

Phospho-p53 (S15) in Human Breast Cancer Tissue. p53 phosphorylated at S15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Immunohistochemistry Phospho-p53 (S15) in Human Breast Cancer Tissue. View Larger

Phospho-p53 (S15) in Human Breast Cancer Tissue. p53 phosphorylated at S15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Human p53 is a 393 amino acid (aa) nuclear/cytoplasmic protein that contains an N-terminal activation domain, a specific DNA binding domain, and a C-terminal domain that mediates tetramer formation and regulates DNA binding. P53 is phosphorylated on Ser15 in response to irradiation, and this phosphorylation increases recruitment of the coactivator CREB-binding protein/p300. The ATM or ATR kinases can phosphorylate p53 at serine 15 (S15), which leads to cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

Product Datasheets

Citations for Human Phospho-p53 (S15) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Paneth Cell Multipotency Induced by Notch Activation following Injury
    Authors: S Yu, K Tong, Y Zhao, I Balasubram, GS Yap, RP Ferraris, EM Bonder, MP Verzi, N Gao
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  2. Skeletal muscle DNA damage precedes spinal motor neuron DNA damage in a mouse model of Spinal Muscular Atrophy (SMA).
    Authors: Fayzullina, Saniya, Martin, Lee J
    PLoS ONE, 2014;9(3):e93329.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  3. Noninvasive assessment of regulable transferred-p53 gene expression and evaluation of therapeutic response with FDG-PET in tumor model.
    Authors: Aung W, Hasegawa S, Koshikawa-Yano M
    Gene Ther., 2010;17(9):1142-51.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. The p53 oncoprotein is a substrate for tissue transglutaminase kinase activity.
    Authors: Mishra S, Murphy LJ
    Biochem. Biophys. Res. Commun., 2005;339(2):726-30.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: WB

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-p53 (S15) Antibody

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Human Phospho-p53 (S15) Antibody
By Anonymous on 07/11/2018
Application: WB Sample Tested: Cancer Cells Species: Human

PC3 cells were treated with UV light for the indicated times. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1mm/ml Human Phospho-p53 (S15) Antibody (AF1043). A specific band was detected for p-P53 at approximately 53kDa. This experiment was conducted under reducing conditions


Human Phospho-p53 (S15) Antibody
By Anonymous on 08/16/2017
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line,T47D human breast cancer cell line Species: Human