The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATR and DNA-PKCS kinases phosphorylate p53 at serine 37 in response to UV light and other genotoxic stressors. This phosphorylation event results in increased p53 transcriptional activation.
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Phosphopeptide containing human p53 S37 site
Specificity
Detects human p53 when phosphorylated at S37. Does not recognize p53 unphosphorylated at S37.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-p53 (S37) Antibody
Detection of Human Phospho-p53 (S37) by Western Blot.
Western blot shows p53 immunoprecipitated from lysates of MCF-7 human breast cancer cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). MCF-7 cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.4 µg/mL Rabbit Anti-Human Phospho-p53 (S37) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3306) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S37) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-p53 (S37) Antibody
Application
Recommended Usage
Western Blot
0.4 µg/mL
Sample: Phospho-p53 (S37) immunoprecipitated from lysates of MCF‑7 human breast cancer cell line treated with camptothecin using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355)
Sample: Phospho-p53 (S37) immunoprecipitated from lysates of MCF‑7 human breast cancer cell line treated with camptothecin using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
Additional p53 Products
Product Documents for Human Phospho-p53 (S37) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Phospho-p53 (S37) Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Apoptosis Signaling Pathway
MAPK Signaling: Oxidative Stress Pathway
mTOR Signaling Pathway
