Detection of Human Phospho-p53 (S46) by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Phospho-p53 (S46) Monoclonal Antibody (Catalog # MAB1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for phospho-p53 (S46) at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
12 months from date of receipt, -20 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 °C under sterile conditions after opening.
The p53 tumor suppressor protein signals apoptosis in cells that have incurred irreparable genotoxic damage. Phosphorylation of p53 at serine 46 is a critical event leading to the cellular decision to undergo apoptosis rather than cell cycle arrest. Serine 46 can be phosphorylated by either the p38 MAP kinase or the Homeodomain-Interacting Protein Kinase 2 (HIPK2).
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