Human Phospho-STAT4 (Y693) Antibody

Catalog # Availability Size / Price Qty
AF4319
AF4319-SP
Human Phospho-STAT4 (Y693) Antibody in Western Blot
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Product Details
Citations (2)
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Human Phospho-STAT4 (Y693) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT4 when phosphorylated at Y693.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Phospho-STAT4 Y693 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-STAT4 (Y693) by Western Blot. View Larger

Detection of Human Phospho-STAT4 (Y693) by Western Blot. Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line untreated (-) or treated (+) with pervanadate for 5 minutes. PVDF membrane was probed with 1 µg/mL Human Phospho-STAT4 (Y693) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4319) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT4 (Y693) was detected at approximately 95 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human/Mouse STAT4 Polyclonal Antibody (lower panel, Catalog # PA-ST4). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

Long Name
Signal Transducer and Activator of Transcription 4
Entrez Gene IDs
6775 (Human); 20849 (Mouse)
Alternate Names
signal transducer and activator of transcription 4; SLEB11; STAT4

Product Datasheets

Citations for Human Phospho-STAT4 (Y693) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Human memory, but not naive, CD4+ T cells expressing transcription factor T-bet might drive rapid cytokine production.
    Authors: Yu S, Zhang Y, Yang B, Wu C
    J Biol Chem, 2014;289(51):35561-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Human invariant natural killer T cells acquire transient innate responsiveness via histone H4 acetylation induced by weak TCR stimulation.
    Authors: Wang X, Bishop KA, Hegde S, Rodenkirch LA, Pike JW, Gumperz JE
    J. Exp. Med., 2012;209(5):987-1000.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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