Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all STAT4 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human Phospho-STAT4 Y693 site

Specificity

Detects human STAT4 when phosphorylated at Y693.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-STAT4 (Y693) Antibody

Detection of Human Phospho-STAT4 (Y693) antibody by Western Blot.

Detection of Human Phospho-STAT4 (Y693) by Western Blot.

Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line untreated (-) or treated (+) with pervanadate for 5 minutes. PVDF membrane was probed with 1 µg/mL Human Phospho-STAT4 (Y693) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4319) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT4 (Y693) was detected at approximately 95 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human/Mouse STAT4 Polyclonal Antibody (lower panel, Catalog # PA-ST4). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-STAT4 (Y693) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NC‑37 human Burkitt's lymphoma B lymphoblast cell line treated with pervanadate

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT4

Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Entrez Gene IDs

6775 (Human); 20849 (Mouse)

Gene Symbol

STAT4

Additional STAT4 Products

Product Documents for Human Phospho-STAT4 (Y693) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Phospho-STAT4 (Y693) Antibody

For research use only

Citations for Human Phospho-STAT4 (Y693) Antibody

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