Human Phospho-STAT4 (Y693) Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human STAT4 when phosphorylated at Y693.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human Phospho-STAT4 Y693 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-STAT4 (Y693) by Western Blot. Western blot shows lysates of NC‑37 human Burkitt's lymphoma B lymphoblast cell line untreated (-) or treated (+) with pervanadate for 5 minutes. PVDF membrane was probed with 1 µg/mL Human Phospho-STAT4 (Y693) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4319) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT4 (Y693) was detected at approximately 95 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0  µg/mL Human/Mouse STAT4 Polyclonal Antibody (lower panel, Catalog # PA-ST4). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT4
Signal transduction and activator of transcription 4 (STAT4) is a member of the Jak/STAT signal transduction pathway. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 is expressed in subset of tissues including spleen, heart, brain, peripheral blood cells and testis. STAT4 is involved in mediating IL-12 response in lymphocytes and regulating T helper cell differentiation.
  • Long Name:
    Signal Transducer and Activator of Transcription 4
  • Entrez Gene IDs:
    6775 (Human); 20849 (Mouse)
  • Alternate Names:
    signal transducer and activator of transcription 4; SLEB11; STAT4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Human memory, but not naive, CD4+ T cells expressing transcription factor T-bet might drive rapid cytokine production.
    Authors: Yu S, Zhang Y, Yang B, Wu C
    J Biol Chem, 2014;289(51):35561-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Human invariant natural killer T cells acquire transient innate responsiveness via histone H4 acetylation induced by weak TCR stimulation.
    Authors: Wang X, Bishop KA, Hegde S, Rodenkirch LA, Pike JW, Gumperz JE
    J. Exp. Med., 2012;209(5):987-1000.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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