Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

R&D Systems | Catalog # DYC4170-2

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Sample Type

Cell Lysates

Reactivity

Human

Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Economical alternative to Western blot
View all VEGFR1/Flt-1 ELISA Kits »

Product Summary for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human VEGF R1 / Flt-1 in cell lysates. An immobilized capture antibody specific for VEGF R1 / Flt-1 binds both phosphorylated and unphosphorylated VEGF R1 / Flt-1. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Kit Contents for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGFR1 (vascular endothelial growth factor receptor 1; Flt-1) binds to VEGF-A, VEGF-B, and PlGF and functions in vasculogenesis, angiogenesis, lymphangiogenesis, and wound healing. It is expressed on endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. It can heterodimerize with VEGFR2 and modulate VEGFR2 mediated endothelial cell proliferation and vessel branching. VEGFR1 engagement on monocyte/macrophage lineage cells enhances their migration, and release of growth factors and cytokines.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

For research use only

Citations for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

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FAQs for Human Phospho-VEGFR1/Flt-1 DuoSet IC ELISA

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  • Q: What are the recommended methods for removal of adherent cells for preparation of cell lysates?

    A: Enzymatic and chemical methods for the removal of cells is not recommended, as they may interfere with the assay. As stated in the datasheet, wash the adherent cells twice with PBS, then add Lysis Buffer #9. Finally, we recommend scraping to remove the adherent cells and resuspend them in the lysis buffer.

  • Q: Which phosphorylated sites are recognized by this assay?

    A: This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

  • Q: What are the recommended methods for removal of adherent cells for preparation of cell lysates?

    A: Enzymatic and chemical methods for the removal of cells is not recommended, as they may interfere with the assay. As stated in the datasheet, wash the adherent cells twice with PBS, then add Lysis Buffer #9. Finally, we recommend scraping to remove the adherent cells and resuspend them in the lysis buffer.

  • Q: Which phosphorylated sites are recognized by this assay?

    A: This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

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