Human Phospholipid Scramblase 1/PLSCR1 Antibody

MAB8137 1 Citation Datasheet / COA / SDS

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MAB8137
MAB8137-SP

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Human Phospholipid Scramblase 1/PLSCR1 Antibody in Western Blot

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospholipid Scramblase 1/PLSCR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospholipid Scramblase 1/PLSCR1 in ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 875327

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Phospholipid Scramblase 1/PLSCR1
Met1-Pro84
Accession # O15162

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human Phospholipid Scramblase 1/PLSCR1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospholipid Scramblase 1/PLSCR1 Monoclonal Antibody (Catalog # MAB8137) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospholipid Scramblase 1/PLSCR1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Phospholipid Scramblase 1/PLSCR1 in HT‑29 Human Cell Line. Phospholipid Scramblase 1/PLSCR1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Phospholipid Scramblase 1/PLSCR1 Monoclonal Antibody (Catalog # MAB8137) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Phospholipid Scramblase 1/PLSCR1

PLSCR1 (phospholipid scramblase 1) is an approximately 37 kDa, type II transmembrane protein that is a member of the Ca2+-dependent phospholipid scramblase family that regulates phospholipid asymmetry of membranes. It is most highly expressed on erythrocytes and platelets, and plays roles in exocytosis and apoptosis. PLSCR1 also has nuclear activity, for example, as an activator of genes in response to interferons. The 318 amino acid (aa) human PLSCR1 contains a 288 aa cytoplasmic domain with three SH3-binding and two WW-binding adaptor domains, three phosphorylation sites, four potential palmitoylation sites (regulating localization to the nucleus or plasma membrane) and a nuclear localization signal, followed by a transmembrane domain and a short extracellular domain. The N-terminus of human PLSCR1 (aa 1-84) shares <60% aa sequence identity with mouse and rat PLSCR1. Potential isoforms have a downstream start site without (311 aa) or with (186 aa) divergence after aa 192, or an upstream start site with divergence of 1 or 11 aa after aa 192 (205, 215 aa).

Entrez Gene IDs

5359 (Human); 22038 (Mouse); 117540 (Rat)

Alternate Names

Erythrocyte phospholipid scramblase; MMTRA1B; MMTRA1BCa(2+)-dependent phospholipid scramblase 1; Phospholipid Scramblase 1; PL scramblase 1; PLSCR1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Phospholipid Scramblase 1/PLSCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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