Detection of Human PI 3‑Kinase p110 gamma by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human PI 3‑Kinase p110 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PI 3‑Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p110 gamma
p110-gamma (protein kinase 110 kDa gamma; also PI3 kinase subunit gamma, Ser/Thr protein kinase PIK3CG and Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma) is a 110-120 kDa Class I member of the PI3/PI4-kinase family of molecules. Schematically, PI3K (PI3 kinase) is typically thought of as a heterodimeric complex composed of one p85, p101 or p84 regulatory subunit coupled to one p110 catalytic subunit. In response to receptor tyrosine kinase ligation, PI3K is recruited to a transmembrane kinase receptor where it is activated. Active PI3K subsequently catalyzes the phosphorylation of phosphatidylinositol, generating a molecule (PIP3) that mediates downstream signaling. p110 gamma is one of four p110 genes that generates PIP3. Unlike p110 alpha, beta and δ, however, it neither interacts with activated Tyr kinases nor with p85. Instead, it associates with either p101 or p84, and interacts with the GPCR G beta gamma subunits to mediate downstream signaling. Human p110 gamma is 1102 amino acids in length. It contains one ABD (adaptor-binding domain) (aa 34-141), an RBD (ras-binding domain) (217-309), a C2 PI3K‑type domain (aa 357-521), a substrate presentating PIK domain (aa 549-725) and a C-terminal catalytic region (aa 830-1046). There is one utilized phosphorylation site at Ser1101. Over aa 298-467, human p110 gamma shares 94% aa sequence identity with mouse p110 gamma.
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