Human PI 3-Kinase p110 gamma Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PI 3‑Kinase p110 gamma in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PI 3‑Kinase p110 gamma
    Asn298-Leu467
    Accession # P48736
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PI 3‑Kinase p110 gamma by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human PI 3‑Kinase p110 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PI 3‑Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p110 gamma

p110-gamma (protein kinase 110 kDa gamma; also PI3 kinase subunit gamma, Ser/Thr protein kinase PIK3CG and Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma) is a 110-120 kDa Class I member of the PI3/PI4-kinase family of molecules. Schematically, PI3K (PI3 kinase) is typically thought of as a heterodimeric complex composed of one p85, p101 or p84 regulatory subunit coupled to one p110 catalytic subunit. In response to receptor tyrosine kinase ligation, PI3K is recruited to a transmembrane kinase receptor where it is activated. Active PI3K subsequently catalyzes the phosphorylation of phosphatidylinositol, generating a molecule (PIP3) that mediates downstream signaling. p110 gamma is one of four p110 genes that generates PIP3. Unlike p110 alpha,  beta and δ, however, it neither interacts with activated Tyr kinases nor with p85. Instead, it associates with either p101 or p84, and interacts with the GPCR G beta gamma subunits to mediate downstream signaling. Human p110 gamma is 1102 amino acids in length. It contains one ABD (adaptor-binding domain) (aa 34-141), an RBD (ras-binding domain) (217-309), a C2 PI3K‑type domain (aa 357-521), a substrate presentating PIK domain (aa 549-725) and a C-terminal catalytic region (aa 830-1046). There is one utilized phosphorylation site at Ser1101. Over aa 298-467, human p110 gamma shares 94% aa sequence identity with mouse p110 gamma.

  • Long Name:
    Protein Tyrosine Phosphatase 1G
  • Entrez Gene IDs:
    5294 (Human); 30955 (Mouse); 298947 (Rat)
  • Alternate Names:
    1-phosphatidylinositol 3-kinase; EC 2.7.1; EC 2.7.1.153; p110-gamma; p120-PI3K; phosphatidylinositol 3 kinase gamma, p110 gamma; phosphatidylinositol 3-kinase catalytic 110-kD gamma; phosphatidylinositol 3-kinase, catalytic, gamma polypeptide; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; phosphoinositide-3-kinase gamma catalytic subunit; phosphoinositide-3-kinase, catalytic, gamma polypeptide; PI 3Kinase p110 gamma; PI 3-Kinase p110 gamma; PI3CG; PI3K; PI3K-gamma ; PI3Kgamma; PI3K-gamma; PI3-kinase subunit gamma; PIK3; PIK3CG; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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