Human PIWIL1/HIWI Antibody Summary
Accession # NP_004755
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
PIWIL1/HIWI in NCI‑N87 Human Cell Line. PIWIL1/HIWI was detected in immersion fixed NCI-N87 human gastric carcinoma cell line using Mouse Anti-Human PIWIL1/HIWI Monoclonal Antibody (Catalog # MAB6548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm in colonies of NCI-N87 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HIWI (human homolog of drosophila piwi), also called PIWIL1 (piwi-like 1) is an 861 amino acid, 99 kDa (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs (piwi domain-binding RNAs). PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34+ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% amino acid sequence identity with mouse and rat PIWIL1.
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