Human PIWIL1/HIWI Antibody

Catalog # Availability Size / Price Qty
PIWIL1/HIWI in NCI‑N87 Human Cell Line.
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Product Details
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Human PIWIL1/HIWI Antibody Summary

Species Reactivity
Detects human PIWIL1/HIWI in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PIWIL2 or rhPIWIL4 is observed.
Monoclonal Mouse IgG1 Clone # 585509
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human PIWIL1/HIWI
Accession # NP_004755
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry PIWIL1/HIWI antibody in NCI-N87 Human Cell Line by Immunocytochemistry (ICC). View Larger

PIWIL1/HIWI in NCI‑N87 Human Cell Line. PIWIL1/HIWI was detected in immersion fixed NCI-N87 human gastric carcinoma cell line using Mouse Anti-Human PIWIL1/HIWI Monoclonal Antibody (Catalog # MAB6548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm in colonies of NCI-N87 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIWIL1/HIWI

HIWI (human homolog of drosophila piwi), also called PIWIL1 (piwi-like 1) is an 861 amino acid, 99 kDa (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs (piwi domain-binding RNAs). PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34+ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% amino acid sequence identity with mouse and rat PIWIL1.

Long Name
Piwi-like Protein 1
Entrez Gene IDs
9271 (Human)
Alternate Names
HIWI; HIWIpiwi homolog; MIWI; piwi (Drosophila)-like 1; PIWI; PIWIL1; piwi-like 1 (Drosophila); piwi-like protein 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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