Human PLC-gamma 1 Antibody

Name: Human PLC-gamma 1 Antibody
Brand: R&D Systems
SKU: AF3288
Price: 479 USD
Availability: InStock

R&D Systems | Catalog # AF3288

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all PLC-gamma 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLC-gamma 1
Ser1091-Leu1290
Accession # P19174

Specificity

Detects human, mouse, and rat PLC-gamma 1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PLC-gamma 1 Antibody

Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat PLC- gamma1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for PLC- gamma1 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human PLC‑ gamma 1 by Simple Western^TM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.1 mg/ml. A specific band was detected for PLC‑ gamma 1 at approximately 150 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Goat Anti-Human/Mouse/Rat PLC‑ gamma 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (043-522-2). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Simple Western electropherogram showing the same Goat Anti-Human/Mouse/Rat PLC‑ gamma 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3288) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the Jurkat human acute T cell leukemia cell line.

Detection of Human PLC-gamma 1 by Simple Western

TIM-3 Association with Intracellular Kinases in CD8+/MART-1+ T cells.TIM-3 co-immunoprecipitation analysis of unactivated and 15 min. stimulation with anti-CD3/CD28 beads (activated). Equivalent amounts of protein (~2mg) were co-immunoprecipitated with pAb anti-TIM-3 antibody and western blot was performed using capillary electrophoresis. Cleared lysate served as a loading control for individual antibody reactivity. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0140694), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human PLC-gamma 1 Antibody

Application

Recommended Usage

Simple Western

2-10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PLC-gamma 1

Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP₃) and diacylglycerol (DAG). IP₃mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.