Detection of Human PP14/Glycodelin by Western Blot.
Western blot shows lysates of human uterus tissue and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human PP14/Glycodelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF842) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PP14/Glycodelin at approximately 24-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Placenta Protein 14 (PP14, also known as Glycodelin-A) is a 28-kDa glycoprotein that is mainly expressed by late secretory-phase endometrium and first-trimester gestational endometrium. It is present at high levels in amniotic fluid, and in the serum of pregnant women. PP14 has been reported to have immuno- suppressive activity that may play a role in fetal survival during pregnancy.
Placental Protein 14
Entrez Gene IDs:
alpha uterine protein; GdF; GdS; Glycodelin; glycodelin-A; glycodelin-F; glycodelin-S; MGC138509; MGC142288; PAEP; PEP; Placental protein 14; PP14 protein (placental protein 14); PP14GDPAEGGdA; pregnancy-associated endometrial a; Pregnancy-associated endometrial alpha-2 globulin; pregnancy-associated endometrial alpha-2-globulin; progestagen-associated endometrial protein (placental protein 14; progestagen-associated endometrial proteinPEG; Progesterone-associated endometrial protein
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