Human PP14/Glycodelin Antibody Summary
Accession # P09466
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PP14/Glycodelin Monoclonal Antibody(Catalog # MAB842).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PP14/Glycodelin by Western Blot. Western blot shows lysates of human uterus tissue and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human PP14/Glycodelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF842) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PP14/Glycodelin at approximately 24-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Placenta Protein 14 (PP14, also known as Glycodelin-A) is a 28-kDa glycoprotein that is mainly expressed by late secretory-phase endometrium and first-trimester gestational endometrium. It is present at high levels in amniotic fluid, and in the serum of pregnant women. PP14 has been reported to have immuno- suppressive activity that may play a role in fetal survival during pregnancy.
Citations for Human PP14/Glycodelin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Affinity purification of native glycodelin from amniotic fluid for biological investigations and development of a glycodelin ELISA for clinical studies
Authors: S Sørensen, V Myrhøj, TH Nguyen, P Aaslo, YB Hansen
Protein Expr. Purif, 2017;130(0):73-80.
Sample Types: Amniotic Fluid
Applications: Western Blot
Glycosylation failure extends to glycoproteins in gestational diabetes mellitus: evidence from reduced alpha2-6 sialylation and impaired immunomodulatory activities of pregnancy-related glycodelin-A.
Authors: Lee CL, Chiu PC, Pang PC, Chu IK, Lee KF, Koistinen R, Koistinen H, Seppala M, Morris HR, Tissot B, Panico M, Dell A, Yeung WS
Sample Types: Amniotic Fluid
Applications: ELISA Development
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