Detection of Human PRDM14 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PRDM14 Monoclonal Antibody (Catalog # MAB6175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRDM14 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PRDM14 in BG01V Human Cell Line.
PRDM14 was detected in fixed BG01V human embryonic stem cells grown on irradiated MEF cells using Human PRDM14 Monoclonal Antibody (Catalog # MAB6175) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400-568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.
PR Domain Containing 14
Entrez Gene IDs:
63978 (Human); 383491 (Mouse)
MGC59730; PFM11; PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing protein 14; PRDM14; PR-domain zinc finger protein 14
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