Detection of Human PREB by Western Blot. |
Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti‑Human PREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5557) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PREB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PREB (Prolactin regulatory element-binding protein; also mSEC12) is a 45 kDa member of the WD-repeat protein family. Although it is ubiquitously expressed, its expression level varies, tissue-to-tissue. It apparently acts as both a DNA-binding transcription factor, and a catalyst for vesicle formation from the ER. Human PREB is a 417 amino acid (aa) type II transmembrane protein. It is found in the ER and nuclear envelope, and may undergo splicing to generate a transcriptional factor. PREB contains a large cytoplasmic region (aa 1‑388) that displays three WD repeats (aa 152‑337), and an eight aa lumenal domain (aa 410‑417). The transcription factor region lies between aa 175‑417. Multiple splice forms exist. There is an alternate start site at Met73 that may be accompanied by either a 47 aa substitution for aa 388‑417, or a 14 aa substitution for aa 409‑417. Over aa 1‑388, human PREB shares 88% aa identity with mouse PREB.
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