Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pregnancy Zone Protein/PZP Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Pregnancy Zone Protein/PZP
Thr26-Val1482
Accession # P20742

Specificity

Detects human Pregnancy Zone Protein/PZP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human A2ML1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Pregnancy Zone Protein/PZP Antibody

Detection of Human Pregnancy Zone Protein/PZP antibody by Western Blot.

Detection of Human Pregnancy Zone Protein/PZP by Western Blot.

Western blot shows human pregnancy serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Pregnancy Zone Protein/PZP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Pregnancy Zone Protein/PZP Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Human pregnancy serum

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pregnancy Zone Protein/PZP

Pregnancy zone protein (PZP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6) is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins.  Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also this protein is found in plasma.  Over aa 26-1482, human PZP is 56% aa identical to mouse PZP.

Alternate Names

CPAMD6, PZP

Entrez Gene IDs

5858 (Human)

Gene Symbol

PZP

UniProt

P20742

Additional Pregnancy Zone Protein/PZP Products

Product Documents for Human Pregnancy Zone Protein/PZP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pregnancy Zone Protein/PZP Antibody

For research use only

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