Detection of Human Pregnancy Zone Protein/PZP by Western Blot.
Western blot shows human pregnancy serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Pregnancy Zone Protein/PZP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pregnancy Zone Protein/PZP
Pregnancy zone protein (PZP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6) is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins. Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also this protein is found in plasma. Over aa 26-1482, human PZP is 56% aa identical to mouse PZP.
Entrez Gene IDs:
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6; CPAMD6; Pregnancy Zone Protein; pregnancy-zone protein; PZP
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