Human/Primate IL-2 Biotinylated Antibody

(8 citations)   
  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human and primate IL-2 in ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL-2
    Ala21-Thr153
    Accession # P60568
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑2 (Catalog # 202-IL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Human/Primate IL-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Primate IL‑2 Antibody (Catalog # MAB602)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate IL‑2 Biotinylated Antibody (Catalog # BAF202)
  • ELISA Standard
     
    Recombinant Human IL-2 Protein (Catalog # 202-IL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL‑2 in Human PBMCs. IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 15 µg/mL Goat Anti-Human/Primate IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
IL‑2 in Human PBMCs. IL‑2 was detected in immersion fixed PMA-, ionomycin-, and monensin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human/Primate IL‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5 - 7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9 - 11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  • References:
    1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
    2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
    3. Taniguchi, T. et al. (1983) Nature 302:305.
    4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
    5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
    6. Wang, X. et al. (2005) Science 310:1159.
    7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
    8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
    9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
    10. Laurence, A. et al. (2007) Immunity 26:371.
    11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
    12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
    13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology
    Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
    Authors: Rochman Y, Yukawa M, Kartashov A, Barski A
    PLoS ONE, 2015;10(4):e0122198.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  3. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  4. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  5. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  6. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  7. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006;172(1):156-65.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  8. Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
    Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
    J. Immunol., 2000;164(10):5337-43.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
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