IL-2 Antibody (5355)

R&D Systems | Catalog # MAB602

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse, Primate - Papio anubis (Olive Baboon), Transgenic Mouse, Xenograft

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Immunocytochemistry

Cited:

Neutralization, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Capture), In vivo assay, Luminex Development, Stimulation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5355
View all IL-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-2
Ala21-Thr153
Accession # NP_000577

Specificity

Detects human and primate IL-2 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-3, rhIL-4, rhIL‑6, rhIL-7, rhIL-8, recombinant mouse (rm) IL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, or rmIL-7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for IL-2 Antibody (5355)

IL‑2 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑2 in Human PBMCs.

IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL-2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Human IL-2 ELISA Standard Curve

Human IL-2 ELISA Standard Curve

Recombinant Human IL‑2 (Catalog # 202-IL) was serially diluted and captured by Mouse Anti-Human/Primate IL‑2 Monoclonal Antibody (Catalog # MAB602) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-202-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for IL-2 Antibody (5355)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin

Human/Primate IL-2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate IL‑2 Biotinylated Antibody (Catalog # BAF202)
  • Standard: Recombinant Human IL-2 Protein (Catalog # 202-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB602 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-2

Interleukin-2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

NP_000577

Additional IL-2 Products

Product Documents for IL-2 Antibody (5355)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for IL-2 Antibody (5355)

For research use only

Citations for IL-2 Antibody (5355)

Customer Reviews for IL-2 Antibody (5355) (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used IL-2 Antibody (5355)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human/Primate IL-2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 08/20/2021
    IL-2 Antibody (5355) MAB602
  • Human/Primate IL-2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Cynomolgus Monkey
    Verified Customer | Posted 12/12/2020
    IL-2 Antibody (5355) MAB602

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies