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Human/Primate IL-2 Antibody

Catalog #: MAB602
31 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB602-SP
MAB602-100
MAB602-500

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IL‑2 in Human PBMCs.
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Product Details
Citations (31)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate IL-2 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IL-2 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-3, rhIL-4, rhIL‑6, rhIL-7, rhIL-8, recombinant mouse (rm) IL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, or rmIL-7 is observed.
Source
Monoclonal Mouse IgG2A Clone # 5355
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-2
Ala21-Thr153
Accession # NP_000577
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Human/Primate IL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate IL‑2 Biotinylated Antibody (Catalog # BAF202)

Standard: Recombinant Human IL-2 Protein (Catalog # 202-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL‑2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑2 in Human PBMCs. IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL-2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

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Citations for Human/Primate IL-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours
    Authors: Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al.
    Transplantation
  2. Cancer-targeted IL-12 controls human rhabdomyosarcoma by senescence induction and myogenic differentiation
    Authors: Karin Schilbach, Mohammed Alkhaled, Christian Welker, Franziska Eckert, Gregor Blank, Hendrik Ziegler et al.
    OncoImmunology
  3. A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism
    Authors: Eleonora Trotta, Paul H. Bessette, Stephanie L. Silveria, Lauren K. Ely, Kevin M. Jude, Duy T. Le et al.
    Nature Medicine
  4. Improved IL-2 immunotherapy by selective stimulation of IL-2 receptors on lymphocytes and endothelial cells
    Authors: Carsten Krieg, Sven Létourneau, Giuseppe Pantaleo, Onur Boyman
    Proceedings of the National Academy of Sciences
  5. Potent antitumour activity of interleukin-2-Fc fusion proteins requires Fc-mediated depletion of regulatory T-cells
    Authors: Rodrigo Vazquez-Lombardi, Claudia Loetsch, Daniela Zinkl, Jennifer Jackson, Peter Schofield, Elissa K. Deenick et al.
    Nature Communications
  6. Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip
    Authors: Girija Goyal, Pranav Prabhala, Gautam Mahajan, Bruce Bausk, Tal Gilboa, Liangxia Xie et al.
    Advanced Science
  7. Sustained Persistence of IL2 Signaling Enhances the Antitumor Effect of Peptide Vaccines through T-cell Expansion and Preventing PD-1 Inhibition
    Authors: Hussein Sultan, Takumi Kumai, Valentyna I. Fesenkova, Aaron E. Fan, Juan Wu, Hyun-Il Cho et al.
    Cancer Immunology Research
  8. Endogenous factors enhance HIV infection of tissue naive CD4 T cells by stimulating high molecular mass APOBEC3G complex formation
    Authors: Jason F. Kreisberg, Wes Yonemoto, Warner C. Greene
    The Journal of Experimental Medicine
  9. Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation
    Authors: Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al.
    Biology of Blood and Marrow Transplantation
  10. Ex vivo blockade of PI3K gamma or delta signaling enhances the antitumor potency of adoptively transferred CD8+ T cells
    Authors: CJ Dwyer, DC Arhontouli, GO Rangel Riv, HM Knochelman, AS Smith, MM Wyatt, MP Rubinstein, C Atkinson, JE Thaxton, DM Neskey, CM Paulos
    Eur. J. Immunol., 2020-05-28;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis
    Authors: BD Bryson, TR Rosebrock, FG Tafesse, CY Itoh, A Nibasumba, GH Babunovic, B Corleis, C Martin, C Keegan, P Andrade, S Realegeno, D Kwon, RL Modlin, SM Fortune
    Nat Commun, 2019-05-27;10(1):2329.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. IL2/anti-IL2 complex combined with CTLA-4, but not PD-1, blockade rescues antitumor NK cell function by regulatory T-cell modulation
    Authors: P Caudana, N Núñez, P DelaRocher, A Pinto, J Denizeau, R Alonso, L Niborski, O Lantz, C Sedlik, E Piaggio
    Cancer Immunol Res, 2019-01-16;0(0):.
    Species: Human
    Sample Types: In Vivo
    Applications: In Vivo
  13. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology
    Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter
    J. Immunol, 2017-03-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Key Residues at Third CDR3? Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs
    Authors: Kenji Chamoto
    J. Immunol, 2016-12-21;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development (Capture)
  15. Rescue of Tolerant CD8+ T Cells during Cancer Immunotherapy with IL2:Antibody Complexes
    Authors: Lauryn E. Klevorn, Melissa M. Berrien-Elliott, Jinyun Yuan, Lindsey M. Kuehm, Gregory D. Felock, Sean A. Crowe et al.
    Cancer Immunology Research
    Species: Xenograft
    Sample Types: In Vivo
    Applications: In vivo assay
  16. Cancer-targeted IL-12 controls human rhabdomyosarcoma by senescence induction and myogenic differentiation
    Authors: Karin Schilbach, Mohammed Alkhaled, Christian Welker, Franziska Eckert, Gregor Blank, Hendrik Ziegler et al.
    OncoImmunology
    Species: Transgenic Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
    Authors: Rochman Y, Yukawa M, Kartashov A, Barski A
    PLoS ONE, 2015-04-10;10(4):e0122198.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  18. Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'.
    Authors: Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
    Nature, 2012-03-25;484(7395):529-33.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  19. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
    Authors: Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW
    Blood, 2008-08-08;112(9):3704-12.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Stimulation
  20. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  21. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  22. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  23. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  24. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Kiryushko D, Pavon L, Novitskaya V, Sandoval-Lopez G, Soroka V, Eugenia Hernandez M, Klingelhofer J, Loria F, Lukanidin E, Estrada I, Berezin V, Perez M, Bock E, Moreno J, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006-02-02;172(1):156-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  25. Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
    Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
    J. Immunol., 2000-05-15;164(10):5337-43.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  26. IL-2/anti-IL-2 antibody complexes show strong biological activity by avoiding interaction with IL-2 receptor alpha subunit CD25
    Authors: Sven Létourneau, Ester M. M. van Leeuwen, Carsten Krieg, Chris Martin, Giuseppe Pantaleo, Jonathan Sprent et al.
    Proceedings of the National Academy of Sciences
  27. Rescue of Tolerant CD8+ T Cells during Cancer Immunotherapy with IL2:Antibody Complexes
    Authors: Lauryn E. Klevorn, Melissa M. Berrien-Elliott, Jinyun Yuan, Lindsey M. Kuehm, Gregory D. Felock, Sean A. Crowe et al.
    Cancer Immunology Research
  28. Ligation of the CD2 co-stimulatory receptor enhances IL-2 production from first-generation chimeric antigen receptor T cells
    Authors: E J Cheadle, D G Rothwell, J S Bridgeman, V E Sheard, R E Hawkins, D E Gilham
    Gene Therapy
  29. Airplug‐Mediated Isolation and Centralization of Single T Cells in Rectangular Microwells for Biosensing
    Authors: Pavithra Sukumar, Muhammedin Deliorman, Ayoola T. Brimmo, Roaa Alnemari, Deena Elsori, Weiqiang Chen et al.
    Advanced Therapeutics
  30. TCB2, a new anti-human interleukin-2 antibody, facilitates heterodimeric IL-2 receptor signaling and improves anti-tumor immunity
    Authors: Jun-Young Lee, Eunjin Lee, Sung-Wook Hong, Daeun Kim, O. Eunju, Jonathan Sprent et al.
    OncoImmunology
  31. Multiplexed Protein Quantification with Barcoded Hydrogel Microparticles
    Authors: David C. Appleyard, Stephen C. Chapin, Patrick S. Doyle
    Analytical Chemistry

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Reviews for Human/Primate IL-2 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Primate IL-2 Antibody
By Anonymous on 08/20/2021
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human/Primate IL-2 Antibody MAB602
Human/Primate IL-2 Antibody
By Anonymous on 12/12/2020
Application: ELISA Sample Tested: Serum Species: Cynomolgus Monkey
ELISA Human/Primate IL-2 Antibody MAB602