Human/Primate IL-2 Antibody

Catalog # Availability Size / Price Qty
MAB602-SP
MAB602-100
MAB602-500
Human/Primate IL-2 Antibody in Data
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Product Details
Citations (14)
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Human/Primate IL-2 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IL-2 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-3, rhIL-4, rhIL‑6, rhIL-7, rhIL-8, recombinant mouse (rm) IL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, or rmIL-7 is observed.
Source
Monoclonal Mouse IgG2A Clone # 5355
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-2
Ala21-Thr153
Accession # NP_000577
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Human/Primate IL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate IL‑2 Biotinylated Antibody (Catalog # BAF202)

Standard: Recombinant Human IL-2 Protein (Catalog # 202-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IL‑2 in Human PBMCs. View Larger

IL‑2 in Human PBMCs. IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL-2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

Citations for Human/Primate IL-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Ex vivo blockade of PI3K gamma or delta signaling enhances the antitumor potency of adoptively transferred CD8+ T cells
    Authors: CJ Dwyer, DC Arhontouli, GO Rangel Riv, HM Knochelman, AS Smith, MM Wyatt, MP Rubinstein, C Atkinson, JE Thaxton, DM Neskey, CM Paulos
    Eur. J. Immunol., 2020;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis
    Authors: BD Bryson, TR Rosebrock, FG Tafesse, CY Itoh, A Nibasumba, GH Babunovic, B Corleis, C Martin, C Keegan, P Andrade, S Realegeno, D Kwon, RL Modlin, SM Fortune
    Nat Commun, 2019;10(1):2329.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. IL2/anti-IL2 complex combined with CTLA-4, but not PD-1, blockade rescues antitumor NK cell function by regulatory T-cell modulation
    Authors: P Caudana, N Núñez, P DelaRocher, A Pinto, J Denizeau, R Alonso, L Niborski, O Lantz, C Sedlik, E Piaggio
    Cancer Immunol Res, 2019;0(0):.
    Species: Human
    Sample Types: In Vivo
    Applications: In Vivo
  4. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology
    Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Key Residues at Third CDR3? Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs
    Authors: Kenji Chamoto
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development (Capture)
  6. Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
    Authors: Rochman Y, Yukawa M, Kartashov A, Barski A
    PLoS ONE, 2015;10(4):e0122198.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  7. Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'.
    Authors: Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
    Nature, 2012;484(7395):529-33.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  8. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
    Authors: Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW
    Blood, 2008;112(9):3704-12.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Stimulation
  9. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  10. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  11. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  12. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  13. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006;172(1):156-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  14. Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
    Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
    J. Immunol., 2000;164(10):5337-43.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

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Secondary Antibodies

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