TRAIL/TNFSF10 Antibody (124723)

R&D Systems | Catalog # MAB3751

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 124723
View all TRAIL/TNFSF10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRAIL
Val114-Gly281
Accession # P50591

Specificity

Detects human and primate TRAIL in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TRAIL/TNFSF10 Antibody (124723)

Human TRAIL / TNFSF10 ELISA Standard Curve

Human TRAIL / TNFSF10 ELISA Standard Curve

Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) was serially diluted and captured by Mouse Anti-Human/Primate TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB3751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for TRAIL/TNFSF10 Antibody (124723)

Human/Primate TRAIL/TNFSF10 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate TRAIL/TNFSF10 Biotinylated Antibody (Catalog # BAF375)
  • Standard: Recombinant Human TRAIL/TNFSF10 Protein (Catalog # 375-TL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL/TNFSF10

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Entrez Gene IDs

8743 (Human); 22035 (Mouse); 246775 (Rat)

Gene Symbol

TNFSF10

UniProt

P50591

Additional TRAIL/TNFSF10 Products

Product Documents for TRAIL/TNFSF10 Antibody (124723)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for TRAIL/TNFSF10 Antibody (124723)

For research use only

Citations for TRAIL/TNFSF10 Antibody (124723)

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