Human TRAIL/TNFSF10 Antibody

Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Human TRAIL/TNFSF10 Antibody.

In the presence of a cross-linking antibody, Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050), and the metabolic inhibitor Actinomycin D, Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375). The ND₅₀is typically

Detection of Human TRAIL/TNFSF10 by Western Blot

IFN-beta -induced TRAIL expression in 2D-cultured cells. To determine the cells that express TRAIL and whose viability would be affected by IFN-beta, 2D-cultured HepG2 cells, primary liver sinusoidal endothelial cells (LSECs), LX-2 human hepatic stellate cells, and macrophages were treated with IFN-beta for 1 day. TRAIL expression was then analyzed via immunoblotting, and cell viability was determined using the WST-1 assay. (A) IFN-beta -induced TRAIL expression in the four cell types. The intensity of protein bands was quantified through densitometry using ImageJ (National Institutes of Health, https://imagej.net/ij/index.html, 10 August 2023), and its relative expression was normalized to that of GAPDH. Data are presented as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001. (B) Cell viability upon IFN-beta treatment in the four cell types. Data are presented as the mean ± SD of three independent experiments. * p < 0.05 and ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/2/1325), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TRAIL/TNFSF10 by Western Blot

Suppression of IFN-beta -induced cell death by JAK inhibitors in liver tumor organoids. Liver tumor organoids were treated with JAK inhibitors (10 µM each of JAK1 and JAK2 inhibitor) for 20 min prior to IFN-beta treatment. After 24 h, the viability of organoids was analyzed using the WST-1 assay, and the expression of TRAIL and cleaved-caspase 3 (CC3) was analyzed by immunoblotting. (A) Viability of liver tumor organoids treated with IFN-beta and/or JAK inhibitors. (B) Expression of TRAIL and cleaved-caspase 3 (CC3) in liver tumor organoids treated with IFN-beta and/or JAK inhibitors. The results are presented as the mean ± standard deviation (SD) of three replicates. * p < 0.05 and *** p < 0.001; inh: inhibitor. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/2/1325), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human TRAIL/TNFSF10 by Western Blot

Cell death in liver tumor organoids induced by ASC-IFN-beta. Adipose-derived stem cells (ASCs) were transiently transfected with pCMV3-IFN-beta. IFN-beta and TRAIL expression was determined via immunoblotting after 1 day. ASC-IFN-beta was added to liver tumor organoids on day 3, and ASC-IFN-beta -induced viability and cell death in liver tumor organoids were examined on day 4. (A) IFN-beta and TRAIL expression in ASC-IFN-beta. The intensity of protein expression was quantified through densitometry using ImageJ (National Institutes of Health, https://imagej.net/ij/index.html, 10 August 2023), and the relative expression was normalized to that of GAPDH. (B) Suppression of liver tumor organoid viability by ASC-IFN-beta. The viability of organoids was analyzed using the WST-1 assay. (C) Cell death induced by ASC-IFN-beta in liver tumor organoids. ASC-IFN-beta -induced cell death was evaluated using a live (calcein-AM) and dead (ethidium homodimer-1) assay. In (C), the white boxes indicate areas that have been enlarged to distinguish the staining of the cells. White arrowheads indicate dead cells. Data shown are representative of three independent experiments. * p < 0.05 and ** p < 0.01. Scale bar: 500 µm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/2/1325), licensed under a CC-BY license. Not internally tested by R&D Systems.