Detects human TRAIL/TNFSF10 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRAIL is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10 (R&D Systems, Catalog # 375-TL) Thr95-Gly281 Accession # P50591
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.03-0.06 µg/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TRAIL/TNFSF10 by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).Specific bands were detected for TRAIL/TNFSF10 at approximately 27 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TRAIL/TNFSF10 in Human Brain. TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Human TRAIL/TNFSF10 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375). The ND50 is typically 0.03-0.06 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TRAIL is a type II transmembrane protein belonging to the TNF
superfamily and is now designated TNFSF10. TRAIL is active as a
homotrimer and is produced by a variety of cell types in both the
membrane bound form and also as a soluble molecule. It binds to any of
the four TRAIL receptors as well as to Osteoprotegerin.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.