Human Prostaglandin E Synthase 2/PTGES2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Prostaglandin E Synthase 2/PTGES2 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Prostaglandin E Synthase 2/PTGES2
    Glu88-His377
    Accession # Q9H7Z7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Prostaglandin E Synthase 2/
PTGES2 by Western Blot.

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostaglandin E Synthase 2/PTGES2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7627) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostaglandin E Synthase 2/PTGES2 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prostaglandin E Synthase 2/PTGES2

PTGES2 (Prostaglandin E Synthase 2; also C9orf15, GBF-1 and mPGES2) is a 32 kDa member of the GST superfamily of molecules. It is a constitutively expressed, integral membrane protein embedded in the Golgi apparatus, and is found in select cell types, including striated muscle cells, neurons, hepatocytes and astrocytes and endothalium. PTGES2 is proposed to lie at the end of a PGE2 synthetic pathway. PLA2S is known to first releases arachidonic acid (AA) from membrane phospholipids. This AA is next converted to PGH2 by COX-1/-2, and the PGH2 is then potentially isomerized into PGE2 by PTGES type enzymes. Notably, PTGES2 is not a glutathione-dependent enzyme, and some evidence suggests it is not a functional prostaglandin synthase. Human PTGES2 is potentially a 377 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 57 aa luminal region, a 17 aa transmembrane segment (aa 58-74) and a 303 aa cytoplasmic domain (aa 75-377). There is one glutaredoxin domain (aa 90-193) and a GST-like region (aa 263-377). Proteolytic cleavage between Ala87Glu88 of 42-43 kDa full-length PTGES2 generates a soluble 32 kDa short form that localizes perinuclearly. There are two potential alternative splice forms. One contains a 19 aa insertion after Ser159, while a second utilizes an alternative start site at Met192. Over aa 88-377, human PTGES2 shares 91% aa sequence identity with mouse PTGES2.

  • Entrez Gene IDs:
    80142 (Human); 96979 (Mouse); 311865 (Rat)
  • Alternate Names:
    C9orf15; chromosome 9 open reading frame 15; EC 5.3.99.3; FLJ14038; gamma-interferon-activated transcriptional element-binding factor 1; GATE-binding factor 1; GBF1; membrane-associated prostaglandin E synthase 2; Microsomal prostaglandin E synthase 2; microsomal prostaglandin E synthase-2; mPGES2; mPGES-2; PGES2; PGES2MGC11289; prostaglandin E synthase 2; PTGES2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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