Human Protein S/PROS1 Antibody

Catalog # Availability Size / Price Qty
AF4036
AF4036-SP
Human Protein S/PROS1 Antibody in Data
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Product Details
Citations (3)
FAQs
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Human Protein S/PROS1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PROS1 in direct ELISAs and Western blots. In these formats, approximately 20% cross‑reactivity with recombinant mouse Protein S is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protein S
Ala42-Trp670
Accession # P07225
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Protein S/PROS1 by Western Blot. View Larger

Detection of Human Protein S/PROS1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Protein S/PROS1 at approximately 80 & 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Protein S/PROS1 by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Protein S/PROS1 by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Protein S/PROS1 at approximately 106 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein S/PROS1

Protein S is a 69 kDa, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins. It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation. Mature human Protein S is 634 amino acids (aa) in length. It contains one Gla domain (aa 42 ‑ 87), four EGF-like repeats (aa 117 - 283) and two laminin G-like domains (aa 299 ‑ 666). Over aa 42 - 670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse, and canine Protein S, respectively.

Entrez Gene IDs
5627 (Human); 19128 (Mouse); 81750 (Rat)
Alternate Names
EC 3.4.21; PROS; PROS1; protein S (alpha); Protein S; protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; vitamin K-dependent plasma protein S; vitamin K-dependent protein S

Product Datasheets

Citations for Human Protein S/PROS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018;515(0):176-190.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  2. Diversification of TAM receptor tyrosine kinase function.
    Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
    Nat Immunol, 2014;15(10):920-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010;115(23):4963-72.
    Species: Mouse
    Sample Types: CSF
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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