Detection of Human Protein S/PROS1 by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Protein S/PROS1 at approximately 80 & 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Protein S/PROS1 by Simple WesternTM.
Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Protein S/PROS1 at approximately 106 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protein S/PROS1
Protein S is a 69 kDa, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins. It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation. Mature human Protein S is 634 amino acids (aa) in length. It contains one Gla domain (aa 42 ‑ 87), four EGF-like repeats (aa 117 - 283) and two laminin G-like domains (aa 299 ‑ 666). Over aa 42 - 670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse, and canine Protein S, respectively.
Entrez Gene IDs:
5627 (Human); 19128 (Mouse); 81750 (Rat)
EC 3.4.21; PROS; PROS1; protein S (alpha); Protein S; protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; vitamin K-dependent plasma protein S; vitamin K-dependent protein S
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