Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 648127
View all Protocadherin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Protocadherin-1
Thr58-Asn162
Accession # Q08174

Specificity

Detects human Protocadherin-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Protocadherin‑1 Antibody

Detection of Protocadherin-1 antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of Protocadherin‑1 in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Protocadherin-1 Monoclonal Antibody (Catalog # MAB5899, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Protocadherin-1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Protocadherin‑1 in BG01V Human Embryonic Stem Cells.

Protocadherin-1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human Protocadherin-1 Monoclonal Antibody (Catalog # MAB5899) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Protocadherin‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protocadherin-1

Protocadherin-1 (PCDH-1; also PC42) is a 150 - 170 kDa delta 1 subgroup member of the nonclustered protocadherin family of molecules. It is expressed on macrophages, respiratory epithelium, endothelial cells and neurons. Protocadherin-1 apparently forms homophilic Ca++-dependent complexes and likely serves as an adhesion molecule. Human full-length Protocadherin-1 precursor is 1237 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 795 aa extracellular domain (ECD) (aa 58-852) plus a 364 aa cytoplasmic region. There are seven cadherin domains in the ECD (aa 58-844), and an RRVTF cytoplasmic motif that binds PP1 alpha phosphatase. There are multiple splice variants. Alternative start sites exist at Met23 and Met235, there is a deletion of aa 209-220, and a 27 aa substitution for aa 1034-1237. Over aa 58-162, human Protocadherin-1 shares 96% aa identity with mouse Protocadherin-1.

Alternate Names

PC42, PCDH1, PCDH42, Protocadherin1

Entrez Gene IDs

5097 (Human); 75599 (Mouse); 307481 (Rat)

Gene Symbol

PCDH1

UniProt

Q08174

Additional Protocadherin-1 Products

Product Documents for Human Protocadherin‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protocadherin‑1 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

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Protocols

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