Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 562615
View all Protocadherin-8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
Lys30-Thr479
Accession # O95206

Specificity

Detects human Protocadherin-8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protocadherin-1, -10, or -15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Protocadherin‑8 Antibody

Protocadherin-8 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

Protocadherin‑8 in MCF‑7 Human Cell Line.

Protocadherin-8 was detected in immersion fixed MCF-7 human breast cancer cell line using Human Protocadherin-8 Monoclonal Antibody (Catalog # MAB5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Protocadherin‑8 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Protocadherin-8

Protocadherin 8 (PCDH-8) is a 160 kDa group F member of the protocadherin family of molecules.  It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca++-dependent homophilic complexes.  In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP.  Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length.  It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region.  There are six cadherin domains in the ECD (aa 30-723).  Multiple splice variants exist.  Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527.  Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Alternate Names

Arcadlin, PAPC, PCDH8, Protocadherin8

Entrez Gene IDs

5100 (Human); 18530 (Mouse); 64865 (Rat)

Gene Symbol

PCDH8

UniProt

O95206

Additional Protocadherin-8 Products

Product Documents for Human Protocadherin‑8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Protocadherin‑8 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

Citations for Human Protocadherin‑8 Antibody

Customer Reviews for Human Protocadherin‑8 Antibody

There are currently no reviews for this product. Be the first to review Human Protocadherin‑8 Antibody and earn rewards!

Have you used Human Protocadherin‑8 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies