Human PTP1B Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human PTP1B in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PTP1B
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

PTP1B in Human Breast Cancer Tissue. PTP1B was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human PTP1B by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and TF‑1 human erythroleukemic cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PTP1B at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human PTP1B by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 57 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1366). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

  • References:
    1. Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
    2. Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
    3. Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
    4. Balsamo, et al. (1996) J. Cell Biol. 134:801.
    5. Elchebly, et al. (1999) Science 283:1544.
  • Long Name:
    Protein Tyrosine Phosphatase 1B
  • Entrez Gene IDs:
    5770 (Human); 19246 (Mouse)
  • Alternate Names:
    EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Inhibition of PTP1B disrupts cell-cell adhesion and induces anoikis in breast epithelial cells
    Authors: B Hilmarsdot, E Briem, S Halldorsso, J Kricker, S Ingthorsso, S Gustafsdot, GM Mælandsmo, MK Magnusson, T Gudjonsson
    Cell Death Dis, 2017;8(5):e2769.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Selective activation of SHP2 activity by cisplatin revealed by a novel chemical probe-based assay.
    Authors: Kuo CC, Chu CY, Lin JJ, Lo LC
    Biochem. Biophys. Res. Commun., 2010;391(1):230-4.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation.
    Authors: Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH, Meng TC
    J. Biol. Chem., 2008;283(50):35265-72.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  4. Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response.
    Authors: Lei K, Townsend DM, Tew KD
    Oncogene, 2008;27(36):4877-87.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  5. Calpain 2 and PTP1B function in a novel pathway with Src to regulate invadopodia dynamics and breast cancer cell invasion.
    Authors: Cortesio CL, Chan KT, Perrin BJ, Burton NO, Zhang S, Zhang ZY, Huttenlocher A
    J. Cell Biol., 2008;180(5):957-71.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Normal Rabbit IgG Control

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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rabbit IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Rabbit IgG (H+L) Antibody

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