Human Rad50 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human Rad50 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Rad50
    Leu518-Leu881
    Accession # Q92878
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Rad50 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Rad50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4996) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad50 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rad50

Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes. It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex. This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells. Human Rad50 is 1312 amino acids (aa) in length. It has an apparent ATP binding site (aa 36‑43) plus a coiled‑coil region (aa 228‑598) followed by a “zinc-hook” domain (aa 635‑734) that mediates homodimerization. There are multiple splice variants. An alternate start site exists at Met140, there is a single Lys substitution for aa 723‑1312, and three Lys substitute for aa 555‑1312. Over aa 518‑881, human Rad50 is 96% aa identical to mouse Rad50.

  • Entrez Gene IDs:
    10111 (Human); 19360 (Mouse); 64012 (Rat)
  • Alternate Names:
    DNA repair protein RAD50; EC 3.6; EC 3.6.1.15; EC 3.6.3; EC 3.6.3.27; hRad50; NBSLD; RAD50 (S. cerevisiae) homolog; RAD50 homolog (S. cerevisiae); Rad50; RAD502; RAD50-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Histone deacetylase inhibitor induces DNA damage, which normal but not transformed cells can repair.
    Authors: Lee JH, Choy ML, Ngo L
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(33):14639-44.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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