Human/Rat/Chicken Laminin S Antibody Summary
Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Laminin S in HeLa Human Cell Line. Laminin S was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Rat/Chicken Laminin S Monoclonal Antibody (Catalog # MAB2066) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin S
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins critically contribute to cell differentation, cell shape and migration and maintenance of tissue phenotypes and survival (1). Laminin S, also known as laminin beta 2, is a subunit of laminin-3 (S-laminin), laminin-4 (S-merosin) and laminin-7 (KS-laminin). It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle.
Citations for Human/Rat/Chicken Laminin S Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Creation of X-linked Alport syndrome rat model with Col4a5 deficiency
Authors: M Namba, T Kobayashi, M Kohno, T Koyano, T Hirose, M Fukushima, M Matsuyama
Scientific Reports, 2021;11(1):20836.
Sample Types: Tissue Lysates
Applications: Western Blot
Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
Authors: Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan
Frontiers in Bioengineering and Biotechnology, 2018;0(0):2296-4185.
Sample Types: Whole Tissue
Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
Authors: Green TL, Hunter DD, Chan W, Merlie JP, Sanes JR
J. Biol. Chem., 1992;267(3):2014-22.
Sample Types: Cell Lysates
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