Human/Rat/Chicken Laminin S Antibody

Catalog # Availability Size / Price Qty
Laminin S in HeLa Human Cell Line.
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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Human/Rat/Chicken Laminin S Antibody Summary

Species Reactivity
Human, Rat, Chicken
Detects human, rat, and chicken Laminin S in Western blots.
Monoclonal Mouse IgG1 Clone # C4
Protein A or G purified from hybridoma culture supernatant
Bovine anterior lens capsule
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot

Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.

Green, T.L. et al. (1992) J. Biol. Chem. 267:2014.
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Laminin S antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Laminin S in HeLa Human Cell Line. Laminin S was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Rat/Chicken Laminin S Monoclonal Antibody (Catalog # MAB2066) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin S

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins critically contribute to cell differentation, cell shape and migration and maintenance of tissue phenotypes and survival (1). Laminin S, also known as laminin beta 2, is a subunit of laminin-3 (S-laminin), laminin-4 (S-merosin) and laminin-7 (KS-laminin). It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle.

  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
Long Name
Laminin, beta 2
Entrez Gene IDs
3913 (Human)
Alternate Names
LAMB2; Laminin B1s chain; Laminin beta 2; Laminin S; laminin subunit beta-2; laminin, beta 2 (laminin S); Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; LAMSLaminin-9 subunit beta; S-LAM beta; S-laminin subunit beta

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Citations for Human/Rat/Chicken Laminin S Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Creation of X-linked Alport syndrome rat model with Col4a5 deficiency
    Authors: M Namba, T Kobayashi, M Kohno, T Koyano, T Hirose, M Fukushima, M Matsuyama
    Scientific Reports, 2021;11(1):20836.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
    Authors: Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan
    Frontiers in Bioengineering and Biotechnology, 2018;0(0):2296-4185.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Authors: Green TL, Hunter DD, Chan W, Merlie JP, Sanes JR
    J. Biol. Chem., 1992;267(3):2014-22.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation


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Human/Rat/Chicken Laminin S Antibody
By Anonymous on 05/18/2022
Application: ICC/IF Sample Tested: HeLa cells Species: Human