Human/Rat Phospho-TrkA (Y785) Antibody

Catalog # Availability Size / Price Qty
Detection of Rat Phospho-TrkA (Y785) by Western Blot.
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Product Details
Citations (2)
Supplemental Products
Reviews (1)

Human/Rat Phospho-TrkA (Y785) Antibody Summary

Species Reactivity
Human, Rat
Detects rat TrkA when phosphorylated at Y785 in Western blots. Reactivity with human Phospho-TrkA is expected due to 100% homology with the immunogen.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing human TrkA Y785 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Phospho-TrkA (Y785) antibody by Western Blot. View Larger

Detection of Rat Phospho-TrkA (Y785) by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with the indicated concentrations of Recombinant Rat beta -NGF (Catalog # 556-NG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human/Rat Phospho-TrkA (Y785) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5479), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-TrkA (Y785) at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

The receptor tyrosine kinase TrkA binds nerve growth factor (NGF) with low affinity, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires a complex formed between TrkA and the p75 NGF receptor. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues, including Y490, Y751 and Y785. Phosphorylation of TrkA at Y785 creates a binding site for phospholipase C-gamma (PLC-gamma ), which catalyzes the cleavage of substrate PIP2 to generate the second messengers DAG and IP3.

Long Name
Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs
4914 (Human); 18211 (Mouse); 59109 (Rat)
Alternate Names
DKFZp781I14186; EC 2.7.10; EC; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

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Citations for Human/Rat Phospho-TrkA (Y785) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
    Authors: Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L
    J Immunol, 2014-02-28;192(7):3345-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Deoxygedunin, a natural product with potent neurotrophic activity in mice.
    Authors: Jang SW, Liu X, Chan CB
    PLoS ONE, 2010-07-13;5(7):e11528.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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Human/Rat Phospho-TrkA (Y785) Antibody
By Anonymous on 10/28/2018
Application: WB Sample Tested: HeLa cells,ZR-75 human breast cancer cell line Species: Human