The receptor tyrosine kinase TrkA binds nerve growth factor (NGF) with low affinity, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires a complex formed between TrkA and the p75 NGF receptor. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues, including Y490, Y751 and Y785. Phosphorylation of TrkA at Y785 creates a binding site for phospholipase C-gamma (PLC-gamma ), which catalyzes the cleavage of substrate PIP2 to generate the second messengers DAG and IP3.
Human/Rat Phospho-TrkA (Y785) Antibody
R&D Systems | Catalog # AF5479
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot, Neutralization Control
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Phosphopeptide containing human TrkA Y785 site
Specificity
Detects rat TrkA when phosphorylated at Y785 in Western blots. Reactivity with human Phospho-TrkA is expected due to 100% homology with the immunogen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Rat Phospho-TrkA (Y785) Antibody
Detection of Rat Phospho-TrkA (Y785) by Western Blot.
Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with the indicated concentrations of Recombinant Rat beta -NGF (Catalog # 556-NG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human/Rat Phospho-TrkA (Y785) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5479), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-TrkA (Y785) at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Rat Phospho-TrkA (Y785) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: PC‑12 rat adrenal pheochromocytoma cell line treated with Recombinant Rat beta ‑NGF (Catalog # 556-NG)
Sample: PC‑12 rat adrenal pheochromocytoma cell line treated with Recombinant Rat beta ‑NGF (Catalog # 556-NG)
Reviewed Applications
Read 2 reviews rated 5 using AF5479 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TrkA
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Alternate Names
NTRK-1, NTRK1
Gene Symbol
NTRK1
Additional TrkA Products
Product Documents for Human/Rat Phospho-TrkA (Y785) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Rat Phospho-TrkA (Y785) Antibody
For research use only
Related Research Areas
Citations for Human/Rat Phospho-TrkA (Y785) Antibody
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Customer Reviews for Human/Rat Phospho-TrkA (Y785) Antibody (2)
5 out of 5
2 Customer Ratings
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Application: Western BlotSample Tested: A2780 ovarian cancer cells and Human prostate cancer cell line (PC3)Species: HumanVerified Customer | Posted 04/04/2020
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Application: Western BlotSample Tested: HeLa cells and ZR-75 human breast cancer cell lineSpecies: HumanVerified Customer | Posted 10/28/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
