Detection of Human RECK by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RECK at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
RECK is a GPI-anchored membrane protein that is highly expressed in
most of the human tissues and untransformed cells, but is undetectable
in tumor cells. RECK can negatively regulate MMP-9, MMP-2 and MTI-MMP
activity and inhibit tumor angiogenesis and metastasis.
Reversion-inducing-cysteine-rich Protein with Kazal Motifs
Entrez Gene IDs:
8434 (Human); 53614 (Mouse)
hRECK; membrane-anchored glycoprotein (metastasis and invasion); RECK; reversion-inducing-cysteine-rich protein with kazal motifs; ST15; ST15reversion-inducing cysteine-rich protein with Kazal motifs; suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein withkazal motifs); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein withkazal motifs); Suppressor of tumorigenicity 15 protein
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