Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RECK Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RECK
Gly27-Val934
Accession # O95980

Specificity

Detects human RECK in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human RECK Antibody

Detection of Human RECK antibody by Western Blot.

Detection of Human RECK by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RECK at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human RECK Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human RECK, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RECK

RECK is a GPI-anchored membrane protein that is highly expressed in most of the human tissues and untransformed cells, but is undetectable in tumor cells. RECK can negatively regulate MMP-9, MMP-2 and MTI-MMP activity and inhibit tumor angiogenesis and metastasis.

Long Name

Reversion-inducing-cysteine-rich Protein with Kazal Motifs

Alternate Names

ST15

Entrez Gene IDs

8434 (Human); 53614 (Mouse)

Gene Symbol

RECK

UniProt

O95980

Additional RECK Products

Product Documents for Human RECK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human RECK Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human RECK Antibody

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